Abstract | BACKGROUND AND OBJECTIVE: MATERIAL AND METHODS: The levels of expression of NEP, ECE-1 and ADAM17 mRNAs in tissue samples collected from the oral buccal mucosal epithelium of 61 patients were investigated by relative quantification using real-time RT-PCR analysis. information on oral and systemic health was obtained from the clinical record of each patient. RESULTS: Among the three groups, classified based on the diagnosis of periodontal diseases (healthy/ gingivitis, early periodontitis and moderate/advanced periodontitis), the relative expression level of NEP mRNA was significantly increased in the early periodontitis group and in the moderate/advanced periodontitis group compared with that in the healthy/ gingivitis group. Moreover, the relative expression levels of ECE1 and ADAM17 mRNAs were significantly increased in the moderate/advanced periodontitis group compared with those in the healthy/ gingivitis group. The correlation coefficients between the mean relative expression levels of NEP and ECE1 mRNAs, NEP and ADAM17 mRNAs, and ECE1 and ADAM17 mRNAs were r = 0.758, r = 0.707 and r = 0.934, respectively (p < 0.001). Furthermore, among the oral-related factors, there was a significant correlation between the number of sites with probing pocket depths of more than 4 mm and of more than 6 mm and the relative expression levels of NEP, ECE1 and ADAM17 mRNAs. In stepwise logistic regression models, high relative expression levels of ECE1 and ADAM17 mRNAs were significantly associated with moderate/advanced periodontitis. CONCLUSION: The present study suggests that the severity of periodontal disease may be associated with the expression of metalloendopeptidase genes, including NEP, ECE1 and ADAM17, in the buccal mucosal epithelium.
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Authors | N Kinoshita, S Awano, A Yoshida, I Soh, T Ansai |
Journal | Journal of periodontal research
(J Periodontal Res)
Vol. 48
Issue 5
Pg. 606-14
(Oct 2013)
ISSN: 1600-0765 [Electronic] United States |
PMID | 23360525
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- Tumor Necrosis Factor-alpha
- Aspartic Acid Endopeptidases
- ADAM Proteins
- Metalloendopeptidases
- Neprilysin
- ECE1 protein, human
- Endothelin-Converting Enzymes
- ADAM17 Protein
- ADAM17 protein, human
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Topics |
- ADAM Proteins
(genetics)
- ADAM17 Protein
- Aged
- Alveolar Bone Loss
(enzymology, genetics)
- Aspartic Acid Endopeptidases
(genetics)
- Chronic Periodontitis
(enzymology, genetics)
- Endothelin-Converting Enzymes
- Epithelium
(enzymology)
- Female
- Gene Expression Regulation, Enzymologic
(genetics)
- Gingival Hemorrhage
(enzymology, genetics)
- Gingivitis
(enzymology, genetics)
- Humans
- Male
- Metalloendopeptidases
(genetics)
- Middle Aged
- Mouth Mucosa
(enzymology)
- Neprilysin
(genetics)
- Periodontal Pocket
(enzymology, genetics)
- Periodontitis
(enzymology, genetics)
- Periodontium
(enzymology)
- Real-Time Polymerase Chain Reaction
(methods)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Tumor Necrosis Factor-alpha
(genetics)
- Young Adult
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