Formulation, characterization and evaluation of an optimized microemulsion formulation of griseofulvin for topical application.

The main objective of the study was to develop a microemulsion (ME) formulation of griseofulvin for the treatment of dermatophytosis (Indian Patent Application 208/DEL/2009). The oil phase was selected on the basis of drug solubility whereas the surfactant and cosurfactant were screened on the basis of their oil solubilizing capacity as well as their efficiency to form ME from pseudo-ternary phase diagrams. The influence of surfactant and cosurfactant mass ratio (Smix) on the ME formation and its permeation through male Laca mice skin was studied. The optimized formulation (ME V) consisting of 0.2% (w/w) griseofulvin, 5% (w/w) oleic acid, 40% (w/w) Smix (1:1, Tween 80 and ethanol) possessed globule size of 12.21 nm, polydispersity index of 0.109 and zeta potential value of -0.139 mV. ME V exhibited 7, 5 and almost 3-fold higher drug permeation as compared to aqueous suspension, oily solution and conventional cream respectively. Besides this the formulation was also evaluated for drug content, pH, stability, dermatopharmacokinetics and antifungal activity against Microsporum canis using guinea pig model for dermatophytosis. Treatment of guinea pigs with ME V resulted in a complete clinical and mycological cure in 7 days. The formulation was observed to be non-sensitizing, histopathologically safe, and stable at 5±3°C, 25±2°C and 40±2°C for a period of six months.
AuthorsNidhi Aggarwal, Shishu Goindi, Ranjit Khurana
JournalColloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 105 Pg. 158-66 (May 1 2013) ISSN: 1873-4367 [Electronic] Netherlands
PMID23357739 (Publication Type: Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2013 Elsevier B.V. All rights reserved.
Chemical References
  • Antifungal Agents
  • Emulsions
  • Oils
  • Surface-Active Agents
  • Water
  • Griseofulvin
  • Administration, Cutaneous
  • Animals
  • Antifungal Agents (pharmacology)
  • Chemistry, Pharmaceutical
  • Emulsions
  • Griseofulvin (chemistry, pharmacology)
  • Guinea Pigs
  • Male
  • Mice
  • Microsporum (drug effects)
  • Oils (chemistry, metabolism)
  • Surface-Active Agents (chemistry, metabolism)
  • Tinea (prevention & control)
  • Water (chemistry, metabolism)
  • Wound Healing (drug effects)

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