HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Regulation of a truncated isoform of AMP-activated protein kinase α (AMPKα) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas.

Abstract
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPKα nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPKα and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia.
AuthorsEric Guévélou, Arnaud Huvet, Rossana Sussarellu, Massimo Milan, Ximing Guo, Li Li, Guofan Zhang, Virgile Quillien, Jean-Yves Daniel, Claudie Quéré, Pierre Boudry, Charlotte Corporeau
JournalJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology (J Comp Physiol B) Vol. 183 Issue 5 Pg. 597-611 (Jul 2013) ISSN: 1432-136X [Electronic] Germany
PMID23354411 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Isoenzymes
  • Citrate (si)-Synthase
  • Proto-Oncogene Proteins c-akt
  • AMP-Activated Protein Kinases
Topics
  • AMP-Activated Protein Kinases (genetics, metabolism)
  • Amino Acid Sequence
  • Animals
  • Citrate (si)-Synthase (metabolism)
  • Crassostrea (metabolism)
  • Hypoxia (metabolism)
  • Isoenzymes (genetics, metabolism)
  • Molecular Sequence Data
  • Muscle, Smooth (metabolism)
  • Muscle, Striated (metabolism)
  • Proto-Oncogene Proteins c-akt (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: