The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.

Abstract

Hepatitis B X-interacting protein (HBXIP) is a novel oncoprotein. In this study, we found that the expression levels of HBXIP were positively associated with those of S-phase kinase-associated protein 2 (Skp2) in clinical breast cancer tissues and cell lines. Moreover, we found that HBXIP was able to stimulate the promoter of Skp2 through binding to the -640/-443 region in Skp2 promoter involving activating E2F transcription factor 1 (E2F1). Skp2 plays crucial roles in HBXIP-enhanced proliferation of breast cancer cells in vitro and in vivo. We conclude that HBXIP up-regulates Skp2 via activating E2F1 to promote proliferation of breast cancer cells.

• Authors: Fuqiang Xu, Xiaona You, Fabao Liu, Xiaohong Shen, Yuanqing Yao, Lihong Ye, Xiaodong Zhang
• Journal: Cancer letters (Cancer Lett) Vol. 333 Issue 1 Pg. 124-32 (Jun 01 2013) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 23352642 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Adaptor Proteins, Signal Transducing
• E2F1 Transcription Factor
• E2F1 protein, human
• LAMTOR5 protein, human
• S-Phase Kinase-Associated Proteins

Topics

• Adaptor Proteins, Signal Transducing (physiology)
• Animals
• Breast Neoplasms (pathology)
• Cell Proliferation
• E2F1 Transcription Factor (metabolism)
• Female
• Humans
• Mice
• Mice, Inbred BALB C
• Promoter Regions, Genetic
• S-Phase Kinase-Associated Proteins (genetics, physiology)
• Up-Regulation