Abstract |
Kaposi's sarcoma (KS) is an infectious cancer occurring most commonly in human immunodeficiency virus (HIV) positive patients and in endemic regions, such as Sub-Saharan Africa, where KS is among the top four most prevalent cancers. The cause of KS is the Kaposi's sarcoma-associated herpesvirus (KSHV, also called HHV-8), an oncogenic herpesvirus that while routinely diagnosed in developed nations, provides challenges to developing world medical providers and point-of-care detection. A major challenge in the diagnosis of KS is the existence of a number of other diseases with similar clinical presentation and histopathological features, requiring the detection of KSHV in a biopsy sample. In this work we develop an answer to this challenge by creating a multiplexed one-pot detection system for KSHV DNA and DNA from a frequently confounding disease, bacillary angiomatosis. Gold and silver nanoparticle aggregation reactions are tuned for each target and a multi-color change system is developed capable of detecting both targets down to levels between 1 nM and 2 nM. The system developed here could later be integrated with microfluidic sample processing to create a final device capable of solving the two major challenges in point-of-care KS detection.
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Authors | Matthew Mancuso, Li Jiang, Ethel Cesarman, David Erickson |
Journal | Nanoscale
(Nanoscale)
Vol. 5
Issue 4
Pg. 1678-86
(Feb 21 2013)
ISSN: 2040-3372 [Electronic] England |
PMID | 23340972
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- DNA, Bacterial
- DNA, Viral
- Silver
- Gold
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Topics |
- Bartonella
(genetics, isolation & purification)
- Colorimetry
(methods)
- DNA, Bacterial
(analysis, genetics)
- DNA, Viral
(analysis, genetics)
- Gold
- Herpesvirus 8, Human
(genetics, isolation & purification)
- Metal Nanoparticles
- Silver
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