Dehydration-induced WRKY genes from tobacco and soybean respond to jasmonic acid treatments in BY-2 cell culture.

Abstract

Drought is one of the important environmental factors affecting crop production worldwide and therefore understanding the molecular response of plant to stress is an important step in crop improvement. WRKY transcription factors are one of the 10 largest transcription factor families across the green lineage. In this study, highly upregulated dehydration-induced WRKY and enzyme-coding genes from tobacco and soybean were selected from microarray data for promoter analyses. Putative stress-related cis-regulatory elements such as TGACG motif, ABRE-like elements; W and G-like sequences were identified by an in silico analyses of promoter region of the selected genes. GFP quantification of transgenic BY-2 cell culture showed these promoters direct higher expression in-response to 100 μM JA treatment compared to 100 μM ABA, 10% PEG and 85 mM NaCl treatments. Thus promoter activity upon JA treatment and enrichment of MeJA-responsive elements in the promoter of the selected genes provides insights for these genes to be jasmonic acid responsive with potential of mediating cross-talk during dehydration responses.

• Authors: Roel C Rabara, Prateek Tripathi, Jun Lin, Paul J Rushton
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 431 Issue 3 Pg. 409-14 (Feb 15 2013) ISSN: 1090-2104 [Electronic] United States
• PMID: 23333328 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Cyclopentanes
• Oxylipins
• Plant Proteins
• Transcription Factors
• jasmonic acid

Topics

• Cells, Cultured
• Cyclopentanes (pharmacology)
• Droughts
• Gene Expression Regulation, Plant
• Oxylipins (pharmacology)
• Plant Proteins (genetics)
• Promoter Regions, Genetic (drug effects, genetics)
• Soybeans (drug effects, genetics)
• Stress, Physiological (genetics)
• Tobacco (drug effects, genetics)
• Transcription Factors (genetics)