Harmaline attenuates voltage--sensitive Ca(2+) currents in neurons of the inferior olive.

Abstract

PURPOSE:

Harmaline is one member of a class of tremorgenic harmala alkaloids that have been implicated in neuroprotective effects and neurodegenerative disorders. It has been reported to interact with several neurotransmitter receptors as well as ion exchangers and voltage-sensitive channels. One site of harmaline action in the brain is the inferior olive (IO). Either local or systemic harmaline injection has been reported to increase spiking rate and coherence in the inferior olive and this activation is thought to produce tremor and ataxia through inferior olivary neuron activation of target neurons in the cerebellum, but the cellular mechanism is not yet known.

METHODS:

Here, we have performed whole cell voltage-clamp and current clamp recordings from olivary neurons in brain slices derived from newborn rats.

RESULTS:

We found that both transient low-voltage activated (LVA) and sustained high voltage-activated (HVA) Ca(2+) currents are significantly attenuated by 0.125 - 0.25 mM harmaline applied to the bath and that this attenuation is partially reversible. In current clamp recordings, spike-afterhyperpolarization complexes were evoked by brief positive current injections. Harmaline produced a small attenuation of spike amplitude, but large spike broadening associated with attenuation of the fast and medium afterhyperpolarization.

CONCLUSION:

Our data suggest that one mode of olivary neuron activation by harmaline involves attenuation of both HVA and LVA Ca(2+) conductances and consequent attenuation of Ca(2+)-sensitive K(+) conductances resulting in spike broadening and attenuation of the afterhyperpolarization. Both of HVA and LVA attenuation also suggests a role to regulate intracelluar Ca(2+), thereby to protect neurons from apoptosis.

• Authors: Xiping Zhan, Werner M Graf
• Journal: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques (J Pharm Pharm Sci) Vol. 15 Issue 5 Pg. 657-68 ( 2012) ISSN: 1482-1826 [Electronic] Canada
• PMID: 23331904 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Calcium Channels
• Potassium Channels, Calcium-Activated
• Harmaline
• Calcium

Topics

• Animals
• Animals, Newborn
• Calcium (metabolism)
• Calcium Channels (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Female
• Harmaline (administration & dosage, pharmacology)
• Male
• Neurons (drug effects, metabolism)
• Olivary Nucleus (drug effects, metabolism)
• Patch-Clamp Techniques
• Potassium Channels, Calcium-Activated (metabolism)
• Rats
• Rats, Sprague-Dawley