Usefulness of cefovecin disk-diffusion test for predicting mecA gene-containing strains of Staphylococcus pseudintermedius and clinical efficacy of cefovecin in dogs with superficial pyoderma.

Abstract

BACKGROUND:

Cefovecin has been widely used to treat skin infections in dogs. The relationship of the cefovecin disk-diffusion test results to the presence of the mecA gene and the clinical efficacy of cefovecin have not been fully evaluated.

HYPOTHESIS/OBJECTIVES:

To determine the usefulness of an in vitro cefovecin disk-diffusion test in predicting the presence of the mecA gene in Staphylococcus pseudintermedius, as well as the in vivo efficacy of cefovecin therapy in dogs with superficial pyoderma.

METHODS:

Twenty-six S. pseudintermedius strains isolated from 22 dogs with pyoderma were used. In vitro disk-diffusion test results of cefovecin were compared with agar-dilution test results, the presence of the mecA gene, and the improvement in clinical scores of dogs with superficial pyoderma at 14 days post treatment.

RESULTS:

There was a significant linear correlation (r = -0.83) between the diameter of the obvious zone of inhibition by disk diffusion and the minimal inhibitory concentration for cefovecin (P < 0.0001). Receiver operating characteristic analysis revealed that zone diameters between 25 and 27 mm exhibited better sensitivity (92.9%) and specificity (100.0%) for detection of strains carrying the mecA gene. The mean improvement in clinical scores in dogs carrying cefovecin-resistant strains was significantly lower than in dogs carrying cefovecin-susceptible strains (P < 0.01).

CONCLUSIONS AND CLINICAL IMPORTANCE:

The cefovecin disk-diffusion test with a cut-off value estimated in this study was valuable for predicting mecA gene carriage in S. pseudintermedius, as well as the in vivo efficacy of cefovecin therapy in dogs with superficial pyoderma caused by S. pseudintermedius.

• Authors: Keita Iyori, Yoichi Toyoda, Kaori Ide, Toshiroh Iwasaki, Koji Nishifuji
• Journal: Veterinary dermatology (Vet Dermatol) Vol. 24 Issue 1 Pg. 162-7.e35-6 (Feb 2013) ISSN: 1365-3164 [Electronic] England
• PMID: 23331693 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 The Authors. Veterinary Dermatology © 2013 ESVD and ACVD.

Chemical References

• Anti-Bacterial Agents
• Bacterial Proteins
• Cephalosporins
• MecA protein, Staphylococcus pseudintermedius
• cefovecin

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Bacterial Proteins (genetics, metabolism)
• Cephalosporins (pharmacology)
• Disk Diffusion Antimicrobial Tests
• Dog Diseases (microbiology)
• Dogs
• Female
• Gene Expression Regulation, Bacterial (physiology)
• Male
• Microbial Sensitivity Tests
• Pyoderma (microbiology, veterinary)
• Staphylococcal Skin Infections (microbiology, veterinary)
• Staphylococcus (drug effects, genetics, metabolism)