Abstract | OBJECTIVE: METHODS: A total of 24 adult male SD rats were divided into 4 groups: (1) control group (n = 6); (2) SAH group (n = 6); (3) SAH + vehicle group (n = 6) and (4) SAH + simvastatin group (n = 6). SAH was induced by injecting 0.3 ml of fresh arterial, non-heparinized blood into prechiasmatic cistern in 20 sec with a syringe pump. In the SAH + simvastatin group, simvastatin was administered ip at a dose of 20 mg×kg(-1)×d(-1) after SAH. Brain samples were excised after perfusion fixation at 7 days post-SAH. Microclots were evaluated by H&E staining. Microthrombi formation was measured by fibrinogen immunostaining. RESULTS: The number of microthrombi was 1 ± 1, 25 ± 5, 24 ± 4 and 5 ± 2 in control, SAH, vehicle and simvastatin groups respectively. The number of microthrombi significantly increased in cerebral cortex at 7 days post-SAH (P < 0.01). The treatment of simvastatin down-regulated the formation of microclots in the SAH model and the number of microthrombi decreased significantly in the SAH + simvastatin group as compared with the SAH or SAH + vehicle groups (P < 0.01). CONCLUSION: The administration of simvastatin alleviates microthrombosis in late phase of SAH in this prechiasmatic blood injection model.
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Authors | Yun Zhu, Gang Chen, Zhong Wang |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 92
Issue 47
Pg. 3374-6
(Dec 18 2012)
ISSN: 0376-2491 [Print] China |
PMID | 23328604
(Publication Type: English Abstract, Journal Article)
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Chemical References |
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Topics |
- Animals
- Disease Models, Animal
- Intracranial Thrombosis
(drug therapy, etiology)
- Male
- Rats
- Rats, Sprague-Dawley
- Simvastatin
(pharmacology, therapeutic use)
- Subarachnoid Hemorrhage
(complications, drug therapy)
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