Abstract |
Bacterial interactions in mixed infections may interfere with antimicrobial therapy. The in-vitro efficacy of ampicillin alone, combination sulbactam/ ampicillin, and metronidazole was studied. Strains of Bacteroides fragilis, Escherichia coli, and Enterococcus faecalis, alone and in association, were tested by means of a broth dilution method. Minimal bactericidal concentrations (MBC) of ampicillin for B. fragilis 74 in association with E. coli 68 were up to 16-fold higher than for B. fragilis 74 alone (256 compared to 16 mg/l), but only 2-fold higher for sulbactam (5 mg/l)/ ampicillin (0.5 and 0.25 g/l). Association of B. fragilis 45 and E. faecalis 186 increased ampicillin MBC of E. faecalis 186 from 2 to 16 mg/l, but the combination sulbactam/ ampicillin restored activity of ampicillin. In association with E. faecalis, metronidazole MBCs of B. fragilis increased up to 64-fold. Strains of E. faecalis and E. coli were able to destroy 10 mg/l metronidazole within 8 to 20 h. The present experiments demonstrated effectiveness of sulbactam/ ampicillin to inhibit beta-lactamases of associated pathogens. Destruction of metronidazole by E. faecalis lends additional support to the use of the combination in aerobic/anaerobic infections including E. faecalis.
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Authors | W R Heizmann, F Heilmann, B Egeler, H Werner |
Journal | Infection
(Infection)
1990 Mar-Apr
Vol. 18
Issue 2
Pg. 117-21
ISSN: 0300-8126 [Print] Germany |
PMID | 2332245
(Publication Type: Journal Article)
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Chemical References |
- Metronidazole
- Ampicillin
- beta-Lactamases
- Sulbactam
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Topics |
- Ampicillin
(therapeutic use)
- Bacteria, Aerobic
(drug effects, growth & development, isolation & purification)
- Bacteria, Anaerobic
(drug effects, growth & development, isolation & purification)
- Bacterial Infections
(drug therapy, microbiology)
- Drug Therapy, Combination
- Humans
- In Vitro Techniques
- Metronidazole
(therapeutic use)
- Microbial Sensitivity Tests
- Sulbactam
(pharmacology, therapeutic use)
- beta-Lactamases
(biosynthesis)
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