[SNP rs16917496 within SET8 3'UTR is associated with the age of onset of breast cancer].

Abstract

OBJECTIVE:

We have identified a SNP within the seed-binding region for miR-502 in the 3'-UTR of the SET8 gene that codes for a methyltransferase for histone H4. SET8 methylates TP53 and thus regulates cell proliferation and genome stability. This study is to investigate the role for this SNP and its interaction with the TP53 codon 72 SNP in the age of onset of breast cancer.

METHODS:

We conducted a case-only study of 1, 110 breast cancer cases. PCR-RFLP was used for SNP genotyping. Ages of onset of breast cancer among different genotypes were analyzed using SAS software.

RESULTS:

Our analysis revealed that the SET8 CC and TP53 GG genotypes were independently associated with earlier age of onset of breast cancer in an allele-dose dependent manner. Moreover, individuals with both SET8 CC and p53 GG genotypes developed cancer at age of 47.74 years, compared with 54.55 years for individuals with both SET8 TT and TP53 CC genotypes.

CONCLUSIONS:

miR-502-binding SNP in SET8 may modulate SET8 expression and contribute to early development of breast cancer either independently or together with the TP53 codon 72 SNP.

• Authors: Bai-lin Zhang, Feng-ju Song, Hong Zheng, Li-na Zhang, Yan-rui Zhao, Ke-xin Chen
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 34 Issue 11 Pg. 835-7 (Nov 2012) ISSN: 0253-3766 [Print] China
• PMID: 23291132 (Publication Type: Journal Article)

Chemical References

• 3' Untranslated Regions
• Codon
• MIRN502 microRNA, human
• MicroRNAs
• Tumor Suppressor Protein p53
• Histone-Lysine N-Methyltransferase
• KMT5A protein, human

Topics

• 3' Untranslated Regions (genetics)
• Adult
• Age of Onset
• Aged
• Aged, 80 and over
• Binding Sites (genetics)
• Breast Neoplasms (genetics)
• Codon
• Female
• Gene Frequency
• Genetic Predisposition to Disease
• Genotype
• Histone-Lysine N-Methyltransferase (genetics)
• Humans
• MicroRNAs (genetics)
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Tumor Suppressor Protein p53 (genetics)
• Young Adult