Leukocyte- and platelet-rich plasma gel (L-PRP gel), a new autologous product which was previously utilized in several
surgical procedures to enhance tissue healing, is now increasingly used as a promising treatment method for
infections. In this study, we investigated the antibacterial property of L-PRP gel against
Methicillin-resistive Staphylococcus aureus (MRSA, ATCC 43300) in a rabbit model of
osteomyelitis. Tibial
osteomyelitis was induced in 40 New Zealand white rabbits using the MRSA strain. Three weeks after induction, the rabbits with tibial
osteomyelitis were randomly divided into four groups: Control group (no treatment); Van group (
debridement and parenteral treatment with
vancomycin alone); L-PRP gel + Van group (
debridement and local L-PRP gel injection, plus parenteral treatment with
vancomycin); L-PRP gel group (
debridement and local L-PRP gel injection). All rabbits were sacrificed 6 weeks after
debridement. The antibacterial efficacy was evaluated by radiological, microbiological, and histological examinations. Newly formed bone was also quantified. The best therapeutic efficacy, including
infection elimination and bone defect repair, was observed in the L-PRP gel + Van group. Although not comparable to
vancomycin, L-PRP gel also exibited antimicrobial efficacy in vivo. We believe that a combination of L-PRP gel and
antibiotics could be a favorable alternative for the treatment of
osteomyelitis.