Invasion into the matrix is one of hallmarks of malignant diseases and is the first step for
tumor metastasis. Thus, analysis of the molecular mechanisms of invasion is essential to overcome
tumor cell invasion. In the present study, we screened for colon
carcinoma-specific genes using a
cDNA microarray database of colon
carcinoma tissues and normal colon tissues, and we found that fermitin family member-1 (FERMT1) is overexpressed in colon
carcinoma cells. FRRMT1, FERMT2 and FERMT3 expression was investigated in colon
carcinoma cells. Reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that only FERMT1 had
cancer cell-specific expression.
Protein expression of FERMT1 was confirmed by western blotting and immunohistochemical staining. To address the molecular functions of FERMT genes in colon
carcinoma cells, we established FERMT1-, FERMT2- and FERMT3-overexpressing colon
carcinoma cells. FERMT1-overexpressing cells exhibited greater invasive ability than did FERMT2- and FERMT3-overexpressing cells. On the other hand, FERMT1-, FERMT2- and FERMT3-overexpressing cells exhibited enhancement of cell growth. Taken together, the results of this study indicate that FERMT1 is expressed specifically in colon
carcinoma cells, and has roles in matrix invasion and cell growth. These findings indicate that FERMT1 is a potential molecular target for
cancer therapy.