Diagnosis of albuminuria by tryptic digestion and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.

Abstract

BACKGROUND:

Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry has been successfully used to detect trace albumin in urine for the diagnosis of albuminuria. However, only the monomeric form of albumin was detected with this approach.

METHODS:

Trypsin was used to digest urinary albumin and its related compounds in urine to produce characteristic peptides. The digest solution was subsequently analyzed by MALDI-TOF MS to obtain peptide ion signals which were used as diagnostic biomarkers for albuminuria.

RESULTS:

The analytical protocol was optimized for efficient digestion and high-performance MALDI-TOF MS analysis. The limit of detection (LOD) of albumin in urine was about 5×10(-7)M.

CONCLUSIONS:

Trypsin digestion combined with MALDI-TOF MS analysis is an efficient and simple approach for rapidly diagnosing albuminuria.

• Authors: Yi-Tzu Cho, Chien-Wen Chen, Miao-Pei Chen, Jia-Lun Hu, Hung Su, Jentaie Shiea, Wen-Jeng Wu, Deng-Chyang Wu
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 420 Pg. 76-81 (May 2013) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 23266772 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Albumins
• Peptides
• Trypsin

Topics

• Albumins (metabolism)
• Albuminuria (diagnosis)
• Humans
• Limit of Detection
• Peptides (urine)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Trypsin (metabolism)