Abstract |
A study is presented on the regulation of alternative splicing (AS) of the Ndufb11 gene of complex I of the mitochondrial respiratory chain and the impact on this process of rotenone treatment in neuroblastoma cells. In physiological conditions the Ndufb11 gene produces at high level a short transcript isoform encoding for a 153 aa protein. This subunit is essential for the assembly of a functional and stable mammalian complex I. The gene produces also, at low level, a longer transcript isoform encoding for a 163 aa protein whose role is unknown. Evidence is presented here showing that the level of the two isoforms is regulated by three DGGGD ESS elements located in exon 2 which can bind the hnRNPH1 protein. In neuronal cells rotenone treatment affects the Ndufb11 alternative splicing pathway, with the increase of the 163/153 mRNAs ratio. This effect appears to be due to the down-regulation of the hnRNPH1 protein. Since rotenone induces apoptosis in neuronal cells, the post-transcriptional regulation of the Ndufb11 gene can be involved in the programmed cell death process.
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Authors | Damiano Panelli, Francesca Paola Lorusso, Francesco Papa, Patrizio Panelli, Alessandro Stella, Massimo Caputi, Anna Maria Sardanelli, Sergio Papa |
Journal | Biochimica et biophysica acta
(Biochim Biophys Acta)
Vol. 1829
Issue 2
Pg. 211-8
(Feb 2013)
ISSN: 0006-3002 [Print] Netherlands |
PMID | 23246602
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
Chemical References |
- NDUFB11 protein, human
- Protein Isoforms
- Rotenone
- Electron Transport Complex I
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Topics |
- Alternative Splicing
(genetics)
- Apoptosis
(drug effects, genetics)
- Cell Line, Tumor
- Electron Transport Complex I
(genetics, metabolism)
- Exons
- Gene Expression Regulation
- Genes, X-Linked
- HEK293 Cells
- HeLa Cells
- Humans
- Neuroblastoma
(genetics, metabolism)
- Protein Isoforms
(genetics)
- Rotenone
(pharmacology)
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