Abstract | RATIONALE: OBJECTIVES: The aim of this study was to characterise temporal changes in elafin concentration in patients with ALI and to evaluate whether a decrease in elafin levels is due to elevated protease activity. METHODS: Bronchoalveolar lavage fluid (BALF) was obtained from patients with ALI within 48 h of onset of ALI (day 0), at day 3 and at day 7. Elafin levels were quantified by ELISA. Elafin susceptibility to proteolytic cleavage by ALI BALF was assessed by Western blot and by high-performance liquid chromatography-mass spectrometry. MEASUREMENTS AND MAIN RESULTS: CONCLUSIONS:
Elafin concentrations fall within the pulmonary compartment over the course of ALI as a result of proteolytic degradation. This loss of elafin may predispose people, in part, to excessive inflammation in ALI.
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Authors | Aoife Kerrin, Sinéad Weldon, Allen Hung-Kang Chung, Thelma Craig, A John Simpson, Cecilia M O'Kane, Danny Francis McAuley, Clifford C Taggart |
Journal | Thorax
(Thorax)
Vol. 68
Issue 4
Pg. 315-21
(Apr 2013)
ISSN: 1468-3296 [Electronic] England |
PMID | 23242946
(Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Elafin
- Recombinant Proteins
- Secretory Leukocyte Peptidase Inhibitor
- Proteasome Endopeptidase Complex
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Topics |
- Acute Lung Injury
(metabolism, physiopathology)
- Blotting, Western
- Bronchoalveolar Lavage Fluid
- Elafin
(analysis, chemistry)
- Electrophoresis, Polyacrylamide Gel
- Humans
- Proteasome Endopeptidase Complex
(pharmacology)
- Proteolysis
(drug effects)
- Recombinant Proteins
(metabolism)
- Secretory Leukocyte Peptidase Inhibitor
(analysis)
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