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Silencing P12CDK²AP¹ with a lentivirus promotes HaCaT cell proliferation.

Abstract
The tumor suppressor P12CDK2AP1 negatively regulates cyclin-dependent kinase 2 (CDK2) activities and suppresses DNA replication. Notably, P12CDK2AP1 is known to be downregulated in head and neck squamous cell carcinomas (HNSCCs). Silencing of specific gene expression by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) using expression vectors and retroviruses has become a powerful tool for the genetic analysis of mammalian cells. In the present study, we utilized lentivirus‑mediated shRNA for functional gene knockdown in normal human skin keratinocytes (HaCaT) cells in order to assess the potential role of P12CDK2AP1 in HNSCCs. Lentivirus‑mediated RNA interference (RNAi) effectively reduced endogenous P12CDK2AP1 expression in HaCaT cells and significantly promoted HaCaT cell proliferation in vitro. Lentiviral vectors have the ability to infect dividing and non-dividing cells as well as to achieve long‑term multilineage gene expression. Thus, additional studies are needed to investigate the use of such vectors as a therapeutic tool for the delivery of siRNAs.
AuthorsMoyi Sun, Jun Zheng, Hui Xue, Yuegui Jiang, Chunnan Li, Jianhu Li, Wei Jin, Minzhi Shen, Xiangming Yang, Qianwei Ni
JournalMolecular medicine reports (Mol Med Rep) Vol. 7 Issue 2 Pg. 471-5 (Feb 2013) ISSN: 1791-3004 [Electronic] Greece
PMID23229879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • CDK2AP1 protein, human
  • RNA, Small Interfering
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase 2
Topics
  • Cell Cycle Checkpoints
  • Cell Line
  • Cyclin-Dependent Kinase 2 (genetics, metabolism)
  • DNA Replication
  • Genetic Vectors (genetics, metabolism)
  • HEK293 Cells
  • Humans
  • Lentivirus (genetics)
  • RNA Interference
  • RNA, Small Interfering (metabolism)
  • Tumor Suppressor Proteins (antagonists & inhibitors, genetics, metabolism)

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