N-WASP is highly expressed in hepatocellular carcinoma and associated with poor prognosis.

Abstract

BACKGROUND:

Neural Wiskott-Aldrich syndrome protein (N-WASP) mediates migration and invasion in cancer cells, but its expression and clinicopathologic and prognostic importance in hepatocellular carcinoma (HCC) remain unknown. The present study was designed to address these issues.

METHODS:

N-WASP expression was first analyzed by Western blotting in 19 paired HCC and paratumoral liver (PTL) tissues. We further evaluated N-WASP expression immunohistochemically in samples from 119 patients with HCC. The clinicopathologic and prognostic importance of N-WASP expression were also investigated.

RESULTS:

Western blotting showed that N-WASP expression was up-regulated in 15 of 19 HCC tissues (79%), compared with PTL ones. The N-WASP-positive rate in immunohistochemical staining also was greater in HCC (63/119, 53%) than that in PTL tissues (8/119, 6%). The up-regulated N-WASP expression in HCC tissues was correlated with absence of capsule formation and predicted less overall and disease-free survival. Multivariate analysis demonstrated that N-WASP was an independent prognostic factor for overall survival and was marginally important for disease-free survival.

CONCLUSION:

These data establish that N-WASP is highly expressed in HCC and its strong prognostic importance. Therefore, the gene/protein might serve as a potential therapeutic target for HCC.

• Authors: Ke-Min Jin, Min Lu, Fang-Fang Liu, Jin Gu, Xiao-Juan Du, Bao-Cai Xing
• Journal: Surgery (Surgery) Vol. 153 Issue 4 Pg. 518-25 (Apr 2013) ISSN: 1532-7361 [Electronic] United States
• PMID: 23218879 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Mosby, Inc. All rights reserved.

Chemical References

• Biomarkers, Tumor
• WASL protein, human
• Wiskott-Aldrich Syndrome Protein, Neuronal

Topics

• Adult
• Aged
• Biomarkers, Tumor (metabolism)
• Blotting, Western
• Carcinoma, Hepatocellular (metabolism, pathology)
• Female
• Humans
• Immunohistochemistry
• Liver Neoplasms (metabolism, pathology)
• Male
• Middle Aged
• Prognosis
• Up-Regulation
• Wiskott-Aldrich Syndrome Protein, Neuronal (metabolism)