Abstract | BACKGROUND: MATERIAL AND METHODS: Rats underwent an acute VHR with one of four synthetic meshes (composite multifilament polyester ( Parietex PCO), multifilament polyester ( Parietex TET), composite monofilament polypropylene (Ventralight), or monofilament polypropylene (SoftMesh)). Prior to implantation, mesh was soaked in saline or 10 mg/ml of vancomycin for 15 min. Following implantation, meshes were contaminated with 10(4) CFU of methicillin-resistant Staphylococcus aureus (MRSA) bacteria. Thirty days after implantation, mesh samples were cultured and evaluated under scanning electron microscope for biofilm formation. RESULTS: Presoaking meshes significantly improves bacterial clearance in composite meshes and multifilament polyester mesh. MRSA clearance was as follows for all meshes (saline-soaked vs. vanco-soaked): Parietex PCO (0 vs. 56 %, p = 0.006), Parietex TET (0 vs. 50 %, p = 0.01), Ventralight (20 vs. 78 %, p = 0.012), and SoftMesh (70 vs. 80 %, p = 0.6). MRSA biofilm formation was consistent with bacterial growth. CONCLUSION: Presoaking multifilament and composite mesh in vancomycin solution reduces MRSA bacterial growth. Its implementation may reduce the risk of mesh infection in clean-contaminated cases, although further investigation with human trials should be performed.
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Authors | Emmanuel E Sadava, David M Krpata, Yue Gao, Yuri W Novitsky, Michael J Rosen |
Journal | Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
(J Gastrointest Surg)
Vol. 17
Issue 3
Pg. 562-8
(Mar 2013)
ISSN: 1873-4626 [Electronic] United States |
PMID | 23212529
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Polyesters
- Polypropylenes
- Vancomycin
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Topics |
- Animals
- Anti-Bacterial Agents
(administration & dosage)
- Biofilms
(drug effects)
- Hernia, Ventral
(surgery)
- Herniorrhaphy
(adverse effects)
- Male
- Methicillin-Resistant Staphylococcus aureus
(drug effects)
- Polyesters
- Polypropylenes
- Prosthesis-Related Infections
(etiology, prevention & control)
- Rats
- Staphylococcal Infections
(microbiology, prevention & control)
- Surgical Mesh
(adverse effects, microbiology)
- Surgical Wound Infection
(etiology, prevention & control)
- Vancomycin
(administration & dosage)
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