The role of immunohistochemistry in the diagnosis of neoplastic pleural effusions.

Abstract

Identification of the origin of neoplastic pleural effusion is a major concern in lung pathology. This study followed the diagnostic role of a panel of antibodies that included calretinin, HBME1, D2-40, Ber-EP4, CK5/6, CEA and TTF1, in a total of 37 cases of pleural and lung cancer with tumor-type cytology, later confirmed by histopathology. For mesothelioma, positive staining for calretinin, D2-40 and CK5/6 and negative for CEA and TTF1 were characteristic. For lung adenocarcinomas, we found Ber-EP4, CEA and TTF1 positivity, and calretinin, D2-40 and CK5/6 negativity. Squamous lung carcinomas were positive for Ber-EP4, CK5/6 and CEA and negative for HBME1, D2-40 and TTF1. The panel of antibodies used in this study provides a differential diagnosis between mesotheliomas and lung carcinomas as well as between lung adenocarcinomas and squamous carcinomas.

• Authors: Mihaela Dinu, Raluca Niculina Ciurea, Mirela Stefan, Ana Claudia Georgescu
• Journal: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (Rom J Morphol Embryol) Vol. 53 Issue 3 Suppl Pg. 817-20 ( 2012) ISSN: 2066-8279 [Electronic] Romania
• PMID: 23188446 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor

Topics

• Biomarkers, Tumor (analysis)
• Cytodiagnosis (methods)
• Humans
• Immunohistochemistry (methods)
• Pleural Effusion, Malignant (diagnosis, pathology)