Abstract | BACKGROUND: METHODS: RESULTS: Sixteen miRNA were significantly differentially expressed in MPNST compared with NF, and of these fourteen were downregulated in MPNST: these included miR-30e*, miR-29c*, miR-29c, miR-340*, miR-30c, miR-139-5p, miR-195, miR-151-5p, miR-342-5p, miR-146a, miR-150, miR-223, let-7 a and let-7 g with a false discovery rate of q=8.48E-03 for the least significant miRNA. In contrast, miR-210 and miR-339-5p were upregulated in MPNST compared with neurofibromas. Prediction softwares/algorithms identified a list of genes targeted by miR-29c including extracellular matrix genes and matrix metalloproteinase (MMP)-2, all of which are reported to be involved in cell migration and invasion. Functional studies in a MPNST cell line, sNF96.2, using a mimic of the mature miR-29c showed reduced invasion, whereas there was no change in proliferation. Zymography of the manipulated cells showed that MMP2 activity was also reduced when miR-29c expression was forced in sNF96.2. CONCLUSION: We provide evidence that reduction of miR-29c has a pivotal role in the progression of nerve sheath tumours and results by increasing the invasive/migratory properties of nerve sheath tumours.
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Authors | N Presneau, M Eskandarpour, T Shemais, S Henderson, D Halai, R Tirabosco, A M Flanagan |
Journal | British journal of cancer
(Br J Cancer)
Vol. 108
Issue 4
Pg. 964-72
(Mar 05 2013)
ISSN: 1532-1827 [Electronic] England |
PMID | 23175151
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN29a microRNA, human
- MicroRNAs
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Topics |
- Cell Line, Tumor
- Disease Progression
- Down-Regulation
- Extracellular Matrix
(genetics)
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Genes, Suppressor
- Humans
- Male
- MicroRNAs
- Middle Aged
- Nerve Sheath Neoplasms
(genetics)
- Neurofibromatosis 1
(genetics)
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