Abstract | AIM: The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133(+) and CD133(-) cells, isolated from primary human colorectal tumors. MATERIALS AND METHODS: After mechanical and enzymatic dissociation of the tumor samples, CD133(+) and CD133(-) subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133(+) and CD133(-) cells isolated from primary and liver metastatic cancer cells. RESULTS: The results indicated that CD133(+) cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133(-) cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133(-) cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. CONCLUSION: The present study provides evidence that CD133(+) cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post- therapy patients.
|
Authors | Marjan Gharagozloo, Hamid R Mirzaei, Bahram Bagherpour, Abbas Rezaei, Hamid Kalantari, Mohammad H Sanei, Mohsen Hosseini, Gholamreza Mohajeri, Abbas Tabatabai, Mozaffar Hashemi |
Journal | Journal of cancer research and therapeutics
(J Cancer Res Ther)
2012 Jul-Sep
Vol. 8
Issue 3
Pg. 399-403
ISSN: 1998-4138 [Electronic] India |
PMID | 23174722
(Publication Type: Journal Article)
|
Chemical References |
- AC133 Antigen
- Antigens, CD
- Biomarkers, Tumor
- Glycoproteins
- PROM1 protein, human
- Peptides
|
Topics |
- AC133 Antigen
- Aged
- Antigens, CD
(metabolism)
- Biomarkers, Tumor
(metabolism)
- Cell Cycle
- Colorectal Neoplasms
(drug therapy, pathology)
- Drug Resistance, Neoplasm
- Female
- Glycoproteins
(metabolism)
- Humans
- Liver Neoplasms
(drug therapy, pathology, secondary)
- Male
- Middle Aged
- Neoplastic Stem Cells
- Peptides
(metabolism)
|