Abstract | SCOPE: There is evidence that a mammalian lignan, enterolactone (ENL), decreases the proliferation rate of prostate cancer cells, although previous studies have used concentrations difficult to achieve through dietary modification. We have therefore investigated the anti-proliferative effects of ENL in an in vitro model of prostate tumourigenesis at concentrations reported to occur in a range of male populations. METHODS AND RESULTS: The effects of 0.1 and 1 μM ENL on three markers of viability and proliferation (metabolic activity, growth kinetics, and cell cycle progression) were assessed in the RWPE-1, WPE1-NA22, WPE1-NB14, WPE1-NB11, WPE1-NB26, LNCaP, and PC-3 cell lines over 72 h. Based on these data, we quantified the expression levels of 12 genes involved in the control of DNA replication initiation using TaqMan real-time PCR in the WPE1-NA22, WPE1-NB14, WPE1-NB11, and WPE1-NB26 cell lines. ENL significantly inhibited the abnormal proliferation of the WPE1-NB14 and WPE1-NB11 cell lines and appears to be a consequence of decreased expression of abnormal chromatin licensing and DNA replication factor 1. CONCLUSION: In contrast to previous studies, concentrations of ENL that are reported after dietary intervention restrict the proliferation of early-stage tumourigenic prostate cell lines by inhibiting the abnormal formation of complexes that initiate DNA replication.
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Authors | Mark J McCann, Ian R Rowland, Nicole C Roy |
Journal | Molecular nutrition & food research
(Mol Nutr Food Res)
Vol. 57
Issue 2
Pg. 212-24
(Feb 2013)
ISSN: 1613-4133 [Electronic] Germany |
PMID | 23148045
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Lignans
- 4-Butyrolactone
- 2,3-bis(3'-hydroxybenzyl)butyrolactone
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Topics |
- 4-Butyrolactone
(analogs & derivatives, pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Cell Transformation, Neoplastic
(pathology)
- DNA Replication
(drug effects)
- Humans
- Lignans
(pharmacology)
- Male
- Mitochondria
(drug effects)
- Prostatic Neoplasms
(drug therapy, pathology)
- Real-Time Polymerase Chain Reaction
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