Anti-proliferative effects of physiological concentrations of enterolactone in models of prostate tumourigenesis.

Abstract

SCOPE:

There is evidence that a mammalian lignan, enterolactone (ENL), decreases the proliferation rate of prostate cancer cells, although previous studies have used concentrations difficult to achieve through dietary modification. We have therefore investigated the anti-proliferative effects of ENL in an in vitro model of prostate tumourigenesis at concentrations reported to occur in a range of male populations.

METHODS AND RESULTS:

The effects of 0.1 and 1 μM ENL on three markers of viability and proliferation (metabolic activity, growth kinetics, and cell cycle progression) were assessed in the RWPE-1, WPE1-NA22, WPE1-NB14, WPE1-NB11, WPE1-NB26, LNCaP, and PC-3 cell lines over 72 h. Based on these data, we quantified the expression levels of 12 genes involved in the control of DNA replication initiation using TaqMan real-time PCR in the WPE1-NA22, WPE1-NB14, WPE1-NB11, and WPE1-NB26 cell lines. ENL significantly inhibited the abnormal proliferation of the WPE1-NB14 and WPE1-NB11 cell lines and appears to be a consequence of decreased expression of abnormal chromatin licensing and DNA replication factor 1.

CONCLUSION:

In contrast to previous studies, concentrations of ENL that are reported after dietary intervention restrict the proliferation of early-stage tumourigenic prostate cell lines by inhibiting the abnormal formation of complexes that initiate DNA replication.

• Authors: Mark J McCann, Ian R Rowland, Nicole C Roy
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 57 Issue 2 Pg. 212-24 (Feb 2013) ISSN: 1613-4133 [Electronic] Germany
• PMID: 23148045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Lignans
• 4-Butyrolactone
• 2,3-bis(3'-hydroxybenzyl)butyrolactone

Topics

• 4-Butyrolactone (analogs & derivatives, pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Cell Transformation, Neoplastic (pathology)
• DNA Replication (drug effects)
• Humans
• Lignans (pharmacology)
• Male
• Mitochondria (drug effects)
• Prostatic Neoplasms (drug therapy, pathology)
• Real-Time Polymerase Chain Reaction