Superoxide generation in different brain regions of rats during normoxia and hypoxia-reoxygenation.
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| Abstract |
The superoxide-dependent chemiluminescent intensity in different brain regions was examined in ex vivo tissue slices of rat brain during normoxia and hypoxia-reoxygenation with lucigenin. The chemiluminescent intensity increased during reoxygenation after hypoxic treatment. There was a higher level of chemiluminescent intensity in the hippocampus during normoxia, and a lower level in the white matter during normoxia and hypoxia-reoxygenation. A weak correlation was found between the chemiluminescent intensity and the glucose uptake rate during normoxia. Then we examined whether hypoxic strength correlates to superoxide generation. The chemiluminescent intensity increased in a hypoxic strength-dependent manner. The generation mechanism of superoxide was examined using carbonyl cyanide m-chlorophenylhydrazone (CCCP), a mitochondrial uncoupler, genipin, an inhibitor for uncoupling protein-2, alloprinol, a xanthine oxidase inhibitor, or apocynin, an NADPH oxidase inhibitor. The chemiluminescent signal was significantly inhibited by CCCP under normoxic condition and enhanced by genipin during normoxia and hypoxia-reoxygenation, but not by allopurinol or apocynin. These results suggest that superoxide generation is high in the hippocampus during normoxia and low in the white matter during normoxia and hypoxia-reoxygenation, superoxide generation in the hypoxia-reoxygenation brain correlates with the strength of hypoxia influenced by oxygen delivery, and mitochondrion is the major sites of intracellular superoxide generation.
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| Authors | Toru Sasaki, Masafumi Yamanaka, Nobuyuki Kagami |
| Journal | Neuroscience research (Neurosci Res) Vol. 74 Issue 3-4 Pg. 261-8 (Dec 2012) ISSN: 1872-8111 [Electronic] Ireland |
| PMID | 23142423 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. |
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