[Effects of matrix metalloproteinases inhibitor GM6001 on choroidal neovascularization].

Abstract

OBJECTIVE:

To explore the efficiency of GM6001 for the inhibition of choroidal neovascularization.

METHODS:

Experimental study. Twenty-four Brown Norvy (BN) rats after photocoagulation were randomly divided into 3 groups as GM6001 group, DMSO group and CONT group. GM6001 (0.2 ml of 0.1% suspension) was injected retrobulbar for GM6001 group and 0.2 ml DMSO was injected for DMSO group on days 1, 3, 6, 9, and 12 after photocoagulation. No injection was performed in the CONT group. Fundus fluorescence angiography, histopathology, immunohistochemistry and quantitative analysis of choroidal neovascularization (CNV) were performed 3 weeks after photocoagulation. One-way ANOVA was used in conjunction with SNK-t test to assess statistical significance within groups.

RESULTS:

The fluorescein leakage appeared in all three groups; but fluorescein leakage of GM6001 group (74.56 ± 2.33) was less than that of DMSO group (119.57 ± 1.15)and CONT group (122.36 ± 2.38) (F = 403.23, P = 0.001; LSD-t test, all P value < 0.01), whereas fluorescein leakage of DMSO group was similar to that in the CONT group. The retinal and choroidal capillaries in the CONT group were damaged and disordered; a great deal of CNV and migration and proliferation of retinal pigment epithelium cells, fibrocytes and collagen fibers were discovered. Pathological changes in DMSO group were similar to those in the CONT group. There were a small quantity of retinal pigment epithelium cells, fibrocytes and CNV in GM6001 group. Although the immunohistochemical staining for CD105 displayed positive results in all three groups, positive staining of GM6001 group (19.85 ± 1.59) was significantly less than that of the CONT group (38.02 ± 2.57) and DMSO group (39.02 ± 3.12) (F = 55.57, P = 0.001; LSD-t test, all P value < 0.01). Positive staining of CD105 in the CONT group was similar to that of DMSO group (P > 0.05). The size of CNV in GM6001 group (15.35 ± 0.77) was significantly less than that of CONT group (28.38 ± 1.60) and DMSO group (28.74 ± 1.19) (F = 114.85, P = 0.001; LSD-t test, all P value < 0.01). There was no statistical difference for the size of CNV between CONT group and DMSO group (P > 0.05).

CONCLUSION:

GM6001 effectively inhibits CVS induced by krypton laser photocoagulation.

• Authors: Xue-guo Chen, Shou-zhi He
• Journal: [Zhonghua yan ke za zhi] Chinese journal of ophthalmology (Zhonghua Yan Ke Za Zhi) Vol. 48 Issue 8 Pg. 744-9 (Aug 2012) ISSN: 0412-4081 [Print] China
• PMID: 23141517 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Dipeptides
• Matrix Metalloproteinase Inhibitors
• N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide
• Matrix Metalloproteinases

Topics

• Animals
• Choroidal Neovascularization (metabolism, pathology, prevention & control)
• Dipeptides (therapeutic use)
• Disease Models, Animal
• Matrix Metalloproteinase Inhibitors (therapeutic use)
• Matrix Metalloproteinases (metabolism)
• Rats
• Rats, Inbred BN