Abstract | OBJECTIVE: To observe effects of electroacupuncture (EA) of "Guanyuan" (GV 4)-"Housanli" (ST 36)-"Baihui" (GV 20,"Shuanggu Yitong" acupoint prescription) on the expression of splenic lymphocyte apoptosis-related genes Fas, Bax, and Bcl-2 in aging rats with yang defiency. METHODS: Thirty Sprague-Dawley aged female rats were randomly and e-ic qually divided into normal control, yang deficiency model (model) and EA groups. The yang deficiency model was established by subcutaneous injection of D-galactose (125 mg/kg) and hydrocortisone(1.5 mg/100 g). EA (2 Hz, 1 mA) was applied to "Guanyuan"(GV 4), "Housanli" (ST 36) and "Baihui" (GV 20) for 15 min, once daily, 6 times per week for 4 weeks. The expression of pro-apoptotic genes Fas, Bax and anti-apoptosis gene Bcl-2 in the spleen tissues were detected by real-time quantitative RT-PCR. RESULTS: In comparison with the normal control group, splenic Fas mRNA and Bax mRNA expression levels were upregulated significantly, and Bcl-2 mRNA expression level was down-regulated considerably in the model group (P < 0. 01). After EA intervention, splenic Fas mRNA and Bax mRNA expression levels were down-regulated markedly, while Bcl-2 mRNA expression up-regulated remarkably in the EA group (P < 0.05, P < 0.01). CONCLUSION: EA of acupoint prescription ["Guanyuan" (GV 4), "Housanli" (ST 36) and "Baihui" (GV 20)] can down-regulate spleen Fas mRNA and Bax mRNA expression, and up-regulate Bcl-2 mRNA expression in aging rats with yang deficiency, which may contribute to its effect in improving yang deficiency in clinic.
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Authors | Hua Wang, Hui-Fang Mao, Jian-Min Liu, Qing Hao, Hong-Tu Tang, Jia Li |
Journal | Zhen ci yan jiu = Acupuncture research
(Zhen Ci Yan Jiu)
Vol. 37
Issue 4
Pg. 266-70
(Aug 2012)
ISSN: 1000-0607 [Print] China |
PMID | 23140045
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoptosis Regulatory Proteins
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Topics |
- Acupuncture Points
- Aging
(genetics, metabolism)
- Animals
- Apoptosis
- Apoptosis Regulatory Proteins
(genetics, metabolism)
- Electroacupuncture
- Female
- Gene Expression
- Humans
- Lymphocytes
(cytology, metabolism)
- Rats
- Rats, Sprague-Dawley
- Spleen
(cytology)
- Yang Deficiency
(genetics, metabolism, physiopathology, therapy)
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