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[Effect of electroacupuncture on spleen lymphocyte apoptosis-related gene expression in aging rats with yang deficiency].

AbstractOBJECTIVE:
To observe effects of electroacupuncture (EA) of "Guanyuan" (GV 4)-"Housanli" (ST 36)-"Baihui" (GV 20,"Shuanggu Yitong" acupoint prescription) on the expression of splenic lymphocyte apoptosis-related genes Fas, Bax, and Bcl-2 in aging rats with yang defiency.
METHODS:
Thirty Sprague-Dawley aged female rats were randomly and e-ic qually divided into normal control, yang deficiency model (model) and EA groups. The yang deficiency model was established by subcutaneous injection of D-galactose (125 mg/kg) and hydrocortisone(1.5 mg/100 g). EA (2 Hz, 1 mA) was applied to "Guanyuan"(GV 4), "Housanli" (ST 36) and "Baihui" (GV 20) for 15 min, once daily, 6 times per week for 4 weeks. The expression of pro-apoptotic genes Fas, Bax and anti-apoptosis gene Bcl-2 in the spleen tissues were detected by real-time quantitative RT-PCR.
RESULTS:
In comparison with the normal control group, splenic Fas mRNA and Bax mRNA expression levels were upregulated significantly, and Bcl-2 mRNA expression level was down-regulated considerably in the model group (P < 0. 01). After EA intervention, splenic Fas mRNA and Bax mRNA expression levels were down-regulated markedly, while Bcl-2 mRNA expression up-regulated remarkably in the EA group (P < 0.05, P < 0.01).
CONCLUSION:
EA of acupoint prescription ["Guanyuan" (GV 4), "Housanli" (ST 36) and "Baihui" (GV 20)] can down-regulate spleen Fas mRNA and Bax mRNA expression, and up-regulate Bcl-2 mRNA expression in aging rats with yang deficiency, which may contribute to its effect in improving yang deficiency in clinic.
AuthorsHua Wang, Hui-Fang Mao, Jian-Min Liu, Qing Hao, Hong-Tu Tang, Jia Li
JournalZhen ci yan jiu = Acupuncture research (Zhen Ci Yan Jiu) Vol. 37 Issue 4 Pg. 266-70 (Aug 2012) ISSN: 1000-0607 [Print] China
PMID23140045 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Apoptosis Regulatory Proteins
Topics
  • Acupuncture Points
  • Aging (genetics, metabolism)
  • Animals
  • Apoptosis
  • Apoptosis Regulatory Proteins (genetics, metabolism)
  • Electroacupuncture
  • Female
  • Gene Expression
  • Humans
  • Lymphocytes (cytology, metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Spleen (cytology)
  • Yang Deficiency (genetics, metabolism, physiopathology, therapy)

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