Abstract | BACKGROUND: In this study the level of IL-23 and IL-27 produced by macrophages derived from peripheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutaneous leishmaniasis lesion were compared before and after treatment with live Leishmania to explore whether IL-23 or IL-27 plays any role in healing process of cutaneous lesions induced by L. major. METHODS: Twenty patients resident in Isfahan Province, with healing or non-healing form of cutaneous leishmaniasis lesion caused by Leishmania major participated in this study. In vitro productions of IL-23 and IL-27 by peripheral blood derived macrophages, before and after stimulation with live L. major (MRHO/IR/75/ER) promastigotes were evaluated using ELISA method. Patient with healing form of lesion received no treatment and patient with non-healing form of lesion received at least 2 courses of glucantime. RESULTS: The mean production of IL-23 and IL-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form of lesion. The levels of IL-23 and IL-27 in culture supernatants before and after stimulation in healing form of CL was significantly higher than non- healing form of CL (P < 0.001). CONCLUSION:
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Authors | S Tolouei, K Ghaedi, A Khamesipour, M Akbari, M Baghaei, Sj Hasheminia, M Narimani, Sh Hejazi |
Journal | Iranian journal of parasitology
(Iran J Parasitol)
Vol. 7
Issue 1
Pg. 18-25
( 2012)
ISSN: 2008-238X [Electronic] Iran |
PMID | 23133467
(Publication Type: Journal Article)
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