Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY:
Magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, has been reported to have anti-inflammatory properties. However, the underlying molecular mechanisms are not well understood. The aim of this study was to investigate the molecular mechanism of magnolol in modifying lipopolysaccharide (LPS)-induced signal pathways in RAW264.7 cells. MATERIAL AND METHODS: RESULTS: The result showed that the purity of magnolol used in this study was 100%. Magnolol inhibited the expression of TNF-α, IL-6 and IL-1β in LPS-stimulated RAW264.7 cells in a dose-dependent manner. Western blot analysis showed that magnolol suppressed LPS-induced NF-κB activation, IκBα degradation, phosphorylation of ERK, JNK and P38. Magnolol could significantly down-regulated the expression of TLR4 stimulating by LPS. Furthermore, magnolol suppressed LPS-induced IL-8 production in HEK293-mTLR4/MD-2 cells. CONCLUSIONS: Our results suggest that magnolol exerts an anti-inflammatory property by down-regulated the expression of TLR4 up-regulated by LPS, thereby attenuating TLR4 mediated the activation of NF-κB and MAPK signaling and the release of pro-inflammatory cytokines. These findings suggest that magnolol may be a therapeutic agent against inflammatory diseases.
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Authors | Yunhe Fu, Bo Liu, Naisheng Zhang, Zhicheng Liu, Dejie Liang, Fengyang Li, Yongguo Cao, Xiaosheng Feng, Xichen Zhang, Zhengtao Yang |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 145
Issue 1
Pg. 193-9
(Jan 09 2013)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 23127653
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Biphenyl Compounds
- Cytokines
- Interleukin-8
- Lignans
- Lipopolysaccharides
- NF-kappa B
- Toll-Like Receptor 4
- magnolol
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Biphenyl Compounds
(pharmacology, therapeutic use)
- Cell Survival
(drug effects)
- Cells, Cultured
- Cytokines
(metabolism)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Gene Expression Regulation
(drug effects)
- HEK293 Cells
- Humans
- Inflammation
(chemically induced, drug therapy)
- Interleukin-8
(metabolism)
- Lignans
(pharmacology, therapeutic use)
- Lipopolysaccharides
- MAP Kinase Signaling System
(drug effects)
- Mice
- NF-kappa B
(antagonists & inhibitors)
- Signal Transduction
(drug effects)
- Toll-Like Receptor 4
(biosynthesis)
- Transfection
(methods)
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