Topical application of a film-forming emulgel dressing that controls the release of stratifin and acetylsalicylic acid and improves/prevents hypertrophic scarring.

Abstract

Here, we evaluate the efficacy of an emulgel dressing to control the release of an antifibrogenic factor, stratifin (SFN), along with an anti-inflammatory drug, acetylsalicylic acid (ASA), to be used as a wound dressing with hypertrophic scar reducing features. Emulgel dressings were prepared by dispersing positively charged submicron vesicles in carboxymethyl cellulose gel. Release kinetics of SFN/ASA and toxicity for primary skin cells were assessed in vitro. Antifibrogenic efficacy of medicated emulgel dressings was tested on a rabbit ear fibrotic model. Following topical application on the wounds, emulgels formed an occlusive film and controlled the release of SFN and ASA for 7 and 24 hours, respectively. Wounds treated with SFN/ASA-containing emulgel dressings showed an 80% reduction in scar elevation compared with untreated controls. Topical formulations were nontoxic for cultured human keratinocytes and fibroblasts. Inflammation was significantly controlled in treated wounds, as shown by a reduced number of infiltrated CD3(+) T cells (p < 0.001) and macrophages. SFN/ASA-treated wounds showed a significantly higher (p < 0.001) expression of matrix metalloproteinase-1, resulting in reduced collagen deposition and less scarring. Film-forming emulgel dressings that control the release of antifibrogenic and anti-inflammatory factors provide an excellent treatment option for postburn hypertrophic scar management.

• Authors: Elham Rahmani-Neishaboor, Reza Jallili, Ryan Hartwell, Victor Leung, Nicholas Carr, Aziz Ghahary
• Journal: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (Wound Repair Regen) 2013 Jan-Feb Vol. 21 Issue 1 Pg. 55-65 ISSN: 1524-475X [Electronic] United States
• PMID: 23126516 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 by the Wound Healing Society.

Chemical References

• 14-3-3 Proteins
• Biomarkers, Tumor
• Delayed-Action Preparations
• Gels
• Exonucleases
• Exoribonucleases
• SFN protein, human
• Matrix Metalloproteinase 1
• Aspirin

Topics

• 14-3-3 Proteins (pharmacology)
• Administration, Cutaneous
• Animals
• Aspirin (administration & dosage, pharmacology)
• Bandages
• Biomarkers, Tumor (pharmacology)
• Cicatrix, Hypertrophic (drug therapy, pathology, prevention & control)
• Delayed-Action Preparations (pharmacology)
• Disease Models, Animal
• Exonucleases (pharmacology)
• Exoribonucleases
• Fibroblasts (drug effects)
• Gels (pharmacology)
• Humans
• Keratinocytes (drug effects)
• Matrix Metalloproteinase 1 (drug effects)
• Rabbits
• Wound Healing (drug effects)
• Wounds and Injuries (drug therapy, pathology)