Abstract | BACKGROUND: METHODS: We performed single left LT in male Sprague-Dawley rats after 3 h of cold ischemia time. H2S donor NaHS (14 μmol/kg, intraperitoneally) or CSE inhibitor propargylglycine (37.5 mg/kg, intraperitoneally) was administered 15 min before the start of the LT. CSE protein expression, H2S generation, and the severity of pulmonary graft injuries were estimated at 24 h after reperfusion. RESULTS: Both CSE protein expression and H2S generation were markedly decreased in transplanted rat lungs compared with those in sham-operated lungs. In the lung-transplanted rats, NaHS administration significantly improved pulmonary function and decreased lipid peroxidation and myeloperoxidase activity. In addition, NaHS inhibited the production of interleukin 1β but increased interleukin 10 levels in graft lung tissues. In contrast, propargylglycine further exacerbated pulmonary function and lung injuries after experimental orthotopic LT. CONCLUSIONS: To our knowledge, this study for the first time has demonstrated that the suppression of CSE expression and H2S production is associated with transplantation-induced lung injury. Both exogenous and endogenous H2S seem to have protective effects against acute LT injury by their multiple functions including antioxidation and anti- inflammation, suggesting that modulation of H2S levels may be considered a potential therapeutic approach in LT.
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Authors | Jingxiang Wu, Jionglin Wei, Xingji You, Xu Chen, Hongwei Zhu, Xiaoyan Zhu, Yujian Liu, Meiying Xu |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 182
Issue 1
Pg. e25-33
(Jun 01 2013)
ISSN: 1095-8673 [Electronic] United States |
PMID | 23122581
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Alkynes
- Enzyme Inhibitors
- Interleukin-1beta
- Sulfides
- Interleukin-10
- propargylglycine
- Cystathionine gamma-Lyase
- sodium bisulfide
- Glycine
- Hydrogen Sulfide
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Topics |
- Alkynes
(pharmacology)
- Animals
- Cold Ischemia
(adverse effects)
- Cystathionine gamma-Lyase
(antagonists & inhibitors, drug effects, metabolism)
- Enzyme Inhibitors
(pharmacology)
- Glycine
(analogs & derivatives, pharmacology)
- Hydrogen Sulfide
(antagonists & inhibitors, metabolism)
- Interleukin-10
(metabolism)
- Interleukin-1beta
(metabolism)
- Lipid Peroxidation
(drug effects, physiology)
- Lung Injury
(etiology, metabolism, physiopathology)
- Lung Transplantation
- Male
- Models, Animal
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(complications)
- Signal Transduction
(drug effects, physiology)
- Sulfides
(pharmacology)
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