Platycodin D attenuates bile duct ligation-induced hepatic injury and fibrosis in mice.

Abstract

Platycodin D (PD) is the major triterpene saponin in the root of Platycodon grandiflorum. The aim of the present study was to evaluate the protective effects of PD on bile duct ligation (BDL)-induced cholestasis in mice. Mice were allocated to five groups: sham, BDL alone, and BDL with PD treatment at 1, 2, and 4mg/kg. PD was administered to the mice for 28 consecutive days after the BDL operation. PD treatment of BDL-operated mice decreased serum alanine aminotransferase, serum aspartate aminotransferase, and total bilirubin levels by up to 37%, 31%, and 41%, respectively, in comparison with the levels in mice that underwent BDL alone. PD treatment attenuated oxidative stress, as evidenced by an increase in anti-oxidative enzyme levels glutathione and superoxide dismutase together with a decrease in lipid peroxidation and oxidative stress indices levels of malondialdehyde and nitric oxide. Histopathological studies further confirmed the protective effects of PD on cholestasis-induced hepatic injury and liver fibrosis in mice. In addition, nuclear factor-kappa B and inducible nitric oxide synthase levels significantly decreased after PD treatment, as did the levels of hepatocyte apoptosis. Taken together, these results suggest that PD treatment might be beneficial in cholestasis-induced hepatotoxicity.

• Authors: Tae-Won Kim, Hong-Ki Lee, In-Bae Song, Jong-Hwan Lim, Eun-Sang Cho, Hwa-Young Son, Ju-Young Jung, Hyo-In Yun
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 51 Pg. 364-9 (Jan 2013) ISSN: 1873-6351 [Electronic] England
• PMID: 23116642 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• NF-kappa B
• Protective Agents
• Saponins
• Triterpenes
• platycodin D
• Superoxide Dismutase
• Aspartate Aminotransferases
• Alanine Transaminase
• Glutathione
• Bilirubin

Topics

• Alanine Transaminase (blood)
• Animals
• Aspartate Aminotransferases (blood)
• Bile Ducts (surgery)
• Bilirubin (blood)
• Cholestasis (pathology, physiopathology, surgery)
• Disease Models, Animal
• Fibrosis (drug therapy)
• Glutathione (blood)
• Ligation
• Liver (drug effects, metabolism, pathology)
• Male
• Mice
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Protective Agents (pharmacology)
• Saponins (pharmacology)
• Superoxide Dismutase (metabolism)
• Triterpenes (pharmacology)