Abstract | BACKGROUND: MATERIAL AND METHODS: Expression profiling of SSTR1-5 was performed on biopsies from 34 GISTs (16 gastric tumors, 15 small intestinal tumors, and three rectal tumors). SSTR scintigraphy ((111)In-octreotide) and measurement of (111)In activity in tumor specimens was performed in seven patients. Uptake and internalization of (177)Lu- octreotate was studied in primary cell cultures from two patients. RESULTS: Quantitative PCR analysis showed expression of SSTR1 and SSTR2 in the majority of tumors, while SSTR3-5 were expressed at low levels. Immunohistochemical analysis confirmed the presence of SSTR1 and SSTR2 proteins in all GISTs, and SSTR3-5 in a subset of tumors. Diagnostic imaging by SSTR scintigraphy, using (111)In-octreotide, demonstrated tumor uptake of (111)In in three of six GIST patients. Measurement of (111)In activity in excised tumor specimens from five patients gave tumor-to-blood (T/B) activity ratios of between eight and 96. Tumor cells in primary culture (gastric and small intestinal GIST) specifically bound and internalized (177)Lu when incubated with the therapeutic compound (177)Lu-octreotate for 4-48 hours (p < 0.05). CONCLUSION:
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Authors | Gabriella Arne, Bengt Nilsson, Johanna Dalmo, Erik Kristiansson, Yvonne Arvidsson, Eva Forssell-Aronsson, Ola Nilsson, Håkan Ahlman |
Journal | Acta oncologica (Stockholm, Sweden)
(Acta Oncol)
Vol. 52
Issue 4
Pg. 783-92
(May 2013)
ISSN: 1651-226X [Electronic] England |
PMID | 23116418
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Radiopharmaceuticals
- Receptors, Somatostatin
- indium-111-octreotide
- Somatostatin
- Lutetium
- Octreotide
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Child
- Female
- Gastrointestinal Neoplasms
(genetics, metabolism)
- Gastrointestinal Stromal Tumors
(genetics, metabolism)
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- Isotope Labeling
- Lutetium
(metabolism)
- Male
- Middle Aged
- Octreotide
(analogs & derivatives, metabolism)
- Protein Binding
- Radiopharmaceuticals
(metabolism)
- Receptors, Somatostatin
(genetics, metabolism)
- Retrospective Studies
- Somatostatin
(analogs & derivatives, metabolism)
- Young Adult
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