Migfilin, α-parvin and β-parvin are differentially expressed in ovarian serous carcinoma effusions, primary tumors and solid metastases.

Abstract

OBJECTIVE:

The objective of this study is to analyze the expression and clinical role of integrin-linked kinase (ILK), α-parvin, β-parvin and migfilin in advanced-stage serous ovarian carcinoma.

METHODS:

Expression of these 4 proteins was investigated in 205 effusions and in 94 patient-matched solid lesions (33 primary tumors and 61 solid metastases) using immunohistochemistry. Protein expression was analyzed for association with clinicopathologic parameters and survival.

RESULTS:

ILK, α-parvin, β-parvin and migfilin were expressed in tumor cells in 53%, 2%, 28% and 53% of effusions and 57%, 20%, 83% and 25% of solid lesions, respectively. Statistical analysis showed significantly higher α-parvin and β-parvin expression in primary carcinomas (p=0.02 and p=0.001, respectively) and solid metastases (p=0.001 and p<0.001, respectively), compared to effusions, with opposite findings for migfilin (p=0.006 and p=0.008 for primary carcinomas and solid metastases, respectively). ILK expression was comparable at all anatomic sites. β-Parvin expression in effusions was associated with better response to chemotherapy at diagnosis (p=0.014), with no other significant association with clinicopathologic parameters or survival. Expression in primary tumors and solid metastases was similarly unrelated to clinicopathologic parameters or survival.

CONCLUSIONS:

This study provides further evidence to our previous observations that the adhesion profile of ovarian serous carcinoma cells in effusions differs from their counterparts in primary carcinomas and solid metastases. β-Parvin may be a novel marker of chemoresponse in metastatic ovarian carcinoma.

• Authors: Ben Davidson, Arild Holth, Mai T P Nguyen, Claes G Tropé, Chuanyue Wu
• Journal: Gynecologic oncology (Gynecol Oncol) Vol. 128 Issue 2 Pg. 364-70 (Feb 2013) ISSN: 1095-6859 [Electronic] United States
• PMID: 23099104 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Cell Adhesion Molecules
• Cytoskeletal Proteins
• FBLIM1 protein, human
• Microfilament Proteins
• PARVA protein, human
• PARVB protein, human
• Actinin

Topics

• Actinin (biosynthesis)
• Adult
• Aged
• Aged, 80 and over
• Ascitic Fluid (metabolism, pathology)
• Cell Adhesion Molecules (biosynthesis)
• Cystadenocarcinoma, Serous (metabolism, pathology)
• Cytoskeletal Proteins (biosynthesis)
• Female
• Humans
• Immunohistochemistry
• Microfilament Proteins (biosynthesis)
• Middle Aged
• Neoplasm Metastasis
• Ovarian Neoplasms (metabolism, pathology)
• Tissue Array Analysis