Abstract |
High-grade gliomas are highly vascularized tumors. Neo-angiogenesis plays a key role in tumor growth and resistance to therapy. A cerebrospinal fluid (CSF) sample could be a useful way to obtain pro-angiogenic predictive or prognostic markers at different stages of the disease. As a first step we looked for pro-angiogenic activity in the CSF of patients with high-grade gliomas. We performed the chicken embryo chorio-allantoic membrane (CAM) assay to study the angiogenic potential of the cerebrospinal fluid (CSF), obtained either by lumbar puncture (LP) or craniotomy from six patients with high-grade brain tumors (three glioblastoma (WHO grade IV), one anaplastic oligodendroglioma (WHO grade III), two anaplastic ganglioglioma (WHO grade III)), and four healthy controls. Significantly increased neo-angiogenesis was observed on the surface of the growing CAM in the 6 patients with high-grade gliomas compared to controls (3.69 ± 1.23 versus 2.16 ± 0.97 capillaries per area (mean ± SD), p<0.005). There was no statistical difference related to the hystological grade of the tumor (WHO grade III or IV), previous treatment (radio- chemotherapy plus temozolomide, temozolomide alone or no treatment), or the site of CSF sample (surgery or lumbar puncture). Our results suggest a pro-angiogenic potential in the CSF of patients with high-grade gliomas.
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Authors | Mariana Sinning, René Letelier, Carlos Rosas, Marcela Fuenzalida, David Lemus |
Journal | Biological research
(Biol Res)
Vol. 45
Issue 2
Pg. 135-8
( 2012)
ISSN: 0717-6287 [Electronic] England |
PMID | 23096357
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adult
- Animals
- Brain Neoplasms
(blood supply, cerebrospinal fluid)
- Case-Control Studies
- Cerebrospinal Fluid
(physiology)
- Chick Embryo
- Chorioallantoic Membrane
(blood supply)
- Craniotomy
- Glioma
(blood supply, cerebrospinal fluid)
- Humans
- Male
- Middle Aged
- Neoplasm Staging
- Neovascularization, Pathologic
(etiology)
- Predictive Value of Tests
- Prognosis
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