Abstract |
The alterations induced in dendritic cells (DCs) in the cancer microenvironment have not been extensively explored. We found that the tumor-associated factor TGF-β may selectively upregulate the expression of miR-27a via the SP1 transcription factor. Importantly, miR-27a altered the activity of NF-κB and MAPKs ( mitogen-activated protein kinases) p38, JNK (c-Jun N-terminal kinases) and ERK ( extracellular signal-regulated kinase 1/2). It influences the production of proinflammatory cytokines by targeting TAB3, p38 MAPK, MAP2K4 and MAP2K7. As a consequence, miR-27a hampered the DC-mediated differentiation of Th1 and Th17 cells in vitro and in vivo, but it promoted the DC-mediated accumulation of Tr1 (CD4(+)IL-10(+)) and Treg (CD4(+)CD25(+)Foxp3(+)) cells in vivo. The repeated infusion of miR-27a-engineered DCs into tumor tissues accelerated tumor growth, indicating that miR-27a is a potential target for tumor immunotherapy.
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Authors | S Min, L Li, M Zhang, Y Zhang, X Liang, Y Xie, Q He, Y Li, J Sun, Q Liu, X Jiang, Y Zhang, Y Che, R Yang |
Journal | Genes and immunity
(Genes Immun)
Vol. 13
Issue 8
Pg. 621-31
(Dec 2012)
ISSN: 1476-5470 [Electronic] England |
PMID | 23034448
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adaptor Proteins, Signal Transducing
- Intracellular Signaling Peptides and Proteins
- MIRN27 microRNA, human
- MicroRNAs
- TAB3 protein, human
- Transforming Growth Factor beta
- Mitogen-Activated Protein Kinases
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Topics |
- Adaptor Proteins, Signal Transducing
- Animals
- Base Sequence
- Cell Communication
- Cell Differentiation
- Cell Line, Tumor
- Dendritic Cells
(drug effects, metabolism, pathology)
- Gene Expression Regulation, Neoplastic
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics, metabolism)
- MAP Kinase Signaling System
- Macrophages
(drug effects, metabolism, pathology)
- Mice
- MicroRNAs
(genetics, metabolism)
- Mitogen-Activated Protein Kinases
(genetics, metabolism)
- Molecular Sequence Data
- Monocytes
(drug effects, metabolism, pathology)
- Oligonucleotide Array Sequence Analysis
- Signal Transduction
- Th1 Cells
(drug effects, metabolism, pathology)
- Th17 Cells
(drug effects, metabolism, pathology)
- Transforming Growth Factor beta
(metabolism, pharmacology)
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