Cytokine expression and the role of Th17 cells in a mouse model of colitis.

Abstract

The aim of this study was to explore the expression of cytokines by Th17 cells and their mechanisms of action in a mouse model of 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced inflammatory bowel disease (IBD). ELISA was used to detect the expression of the Th17 cytokine interleukin, (IL)-17, and that of the Th1 cytokine, interferon-γ (IFN-γ), in colon tissues. Western blot analysis was used to detect IL-17 expression in the peripheral blood mononuclear cells (PBMCs), spleen mononuclear cells (SMCs), mesenteric lymph node cells and colon tissues of the colitic mice. RT-PCR analysis was used to detect the effect of anti-IL-17 antibody application on the tumor necrosis factor (TNF)-α, IFN-γ and IL-6 mRNA levels in the SMCs of the colitic mice. The Th17 cytokine, IL-17, and the Th1 cytokine, IFN-γ, were expressed at high levels in the TNBS-induced colitic mice. In addition, the expression of the Th17 cytokine appeared earlier than that of the Th1 cytokine. The IL-17 levels in the SMCs, mesenteric lymph node cells and colon tissues of the disease model group were significantly different from those of the normal control group (p<0.01), while the IL-17 levels in the PBMCs of the disease model group were not significantly different (p>0.05) from those of the control group. Following the application of 10 µg/ml anti-IL-7 antibody, the TNF-α, IL-6 and IFN-γ mRNA levels in the SMCs of the model group demonstrated no significant differences from those of the non-antibody-treated control group (p>0.05). In conclusion, Th17 and Th1 cells are involved in TNBS-induced IBD and the effect of the Th17 cells may be mediated through the induction of the secretion of pro-inflammatory cytokines.

• Authors: Ying He, Lian-Jie Lin, Chang-Qing Zheng, Yu Jin, Yan Lin
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 6 Issue 6 Pg. 1438-42 (12 2012) ISSN: 1791-3004 [Electronic] Greece
• PMID: 23027085 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Cytokines
• Interleukin-17
• Interleukin-6
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Interferon-gamma
• Trinitrobenzenesulfonic Acid

Topics

• Animals
• Antibodies (pharmacology)
• Colitis (chemically induced, metabolism, pathology)
• Colon (metabolism)
• Cytokines (genetics, metabolism)
• Disease Models, Animal
• Female
• Interferon-gamma (genetics, metabolism)
• Interleukin-17 (immunology, metabolism)
• Interleukin-6 (genetics, metabolism)
• Leukocytes, Mononuclear (drug effects, metabolism)
• Lymph Nodes (cytology)
• Mice
• Mice, Inbred BALB C
• RNA, Messenger (metabolism)
• Spleen (cytology)
• Th17 Cells (immunology, metabolism)
• Trinitrobenzenesulfonic Acid (toxicity)
• Tumor Necrosis Factor-alpha (genetics, metabolism)