Monoclonal antibody to 2-amino-3-methylimidazo(4,5-f)quinoline, a dietary carcinogen.

Abstract

In order to investigate relationships between human carcinogenesis and dietary carcinogens, one hybridoma cell line secreting a monoclonal antibody against 2-amino-3-methylimidazo(4,5-f)quinoline (IQ), a dietary carcinogen, was produced by fusing splenocytes from Balb/c mice immunized with IQ-Lysine(Lys)-Ascaris protein conjugate. The subclass of monoclonal anti-IQ antibody was determined by double immunodiffusion using culture medium and identified as IgG1. Monoclonal anti-IQ antibody was purified from ascites fluids of Balb/c mice with affinity chromatography on Protein A-Sepharose CL4B and analyzed concerning its cross-reactivity and sensitivity with RIA. Finally, we showed that our monoclonal antibody recognized IQ, 2-amino-3,4-dimethylimidazo(4,5-f)quinoline (MeIQ) and several beta-carbolines more intensely and that the sensitivity to IQ was 23 nmol in 50% displacement.

• Authors: H Yanagisawa, T Tachikawa, S Shin, O Wada
• Journal: Applied biochemistry and biotechnology (Appl Biochem Biotechnol) Vol. 23 Issue 1 Pg. 1-13 (Jan 1990) ISSN: 0273-2289 [Print] United States
• PMID: 2301946 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens
• Carbolines
• Carcinogens
• Immunoglobulin G
• Quinolines
• 2-amino-3-methylimidazo(4,5-f)quinoline
• 2-amino-3,4-dimethylimidazo(4,5-f)quinoline

Topics

• Animals
• Antibodies, Monoclonal (biosynthesis, immunology, isolation & purification)
• Antibody Specificity
• Antigens (immunology)
• Ascitic Fluid (immunology)
• Carbolines (immunology)
• Carcinogens
• Chromatography, Affinity
• Diet
• Hybridomas (immunology)
• Immunoglobulin G (immunology)
• Mice
• Mice, Inbred BALB C
• Molecular Weight
• Quinolines (immunology)
• Radioimmunoassay