Abstract | BACKGROUND: Eosinophilic inflammation is implicated in asthma. Eotaxin 1-3 regulate eosinophil trafficking into the airways along with other chemotactic factors. However, the epithelial and bronchoalveolar lavage (BAL) cell expression of these chemokines in relation to asthma severity and eosinophilic phenotypes has not been addressed. OBJECTIVE: To measure the expression of the three eotaxin isoforms in bronchoscopically obtained samples and compare them with clinically relevant parameters between normal subjects and patients with asthma. METHODS: Normal subjects and patients with asthma of varying severity recruited through the Severe Asthma Research Program underwent clinical assessment and bronchoscopy with airway brushing and BAL. Eotaxin 1-3 mRNA/ protein were measured in epithelial and BAL cells and compared with asthma severity, control and eosinophilic inflammation. RESULTS: CONCLUSIONS: Epithelial eotaxin-2 and 3 are increased in asthma and severe asthma. Their expression may contribute to luminal migration of eosinophils, especially in later onset disease, asthma control and severity.
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Authors | John M Coleman, Chetan Naik, Fernando Holguin, Anuradha Ray, Prabir Ray, John B Trudeau, Sally E Wenzel |
Journal | Thorax
(Thorax)
Vol. 67
Issue 12
Pg. 1061-6
(Dec 2012)
ISSN: 1468-3296 [Electronic] England |
PMID | 23015684
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Adrenal Cortex Hormones
- CCL26 protein, human
- Chemokine CCL11
- Chemokine CCL24
- Chemokine CCL26
- Chemokines, CC
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Topics |
- Adrenal Cortex Hormones
(pharmacology)
- Adult
- Age of Onset
- Asthma
(metabolism)
- Bronchoalveolar Lavage
- Bronchoscopy
- Chemokine CCL11
(metabolism)
- Chemokine CCL24
(metabolism)
- Chemokine CCL26
- Chemokines, CC
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Eosinophilia
(metabolism)
- Epithelial Cells
(metabolism)
- Female
- Humans
- Male
- Real-Time Polymerase Chain Reaction
- Statistics, Nonparametric
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