Doxycycline-loaded β-tricalcium phosphate release following EDTA root surface etching improved the clinical outcomes in chronic periodontitis: an in vivo study.

Abstract

BACKGROUND:

The main objective of the present study is to quantify doxycycline (DOX) release from β-tricalcium phosphate (β-TCP) after EDTA root surface treatment.

METHODS:

Thirty systemically healthy patients with ≥1 paired contralateral interproximal intrabony defect ≥4 mm deep along with an interproximal probing depth ≥6 mm and clinical attachment level ≥4 mm were randomized into two groups. Group 1 (G1) consisted of sites treated with open flap debridement followed by placement of DOX blended with β-TCP (DOX-β-TCP), whereas group 2 (G2) sites were treated with flap surgery followed by the placement of DOX blended with β-TCP after EDTA etching of the exposed root surfaces (DOX-β-TCP + EDTA). Samples of gingival crevicular fluid (GCF) were obtained 1, 3, 7, 14, and 21 days after surgery. Quantitative measurements of DOX were taken with high-performance liquid chromatography. Clinical evaluation and follow-up for 6 months were performed.

RESULTS:

At 21 days, the DOX-β-TCP + EDTA-treated group showed a 194.7 µg/mL value. The DOX-β-TCP + EDTA-treated group retained more DOX during the periods of 3, 7, 10, 14, and 21 days than the DOX-β-TCP-treated group. Six months after therapy, DOX-β-TCP + EDTA-treated sites showed more significant clinical improvements compared to DOX-β-TCP-treated sites (P ≤ 0.05).

CONCLUSIONS:

EDTA root surface etching enhances DOX availability in the GCF following its release from β-TCP as a drug carrier.

• Authors: Ahmed Y Gamal, Radi M Kumper, Abd El Reheem R Al Gendy
• Journal: Journal of periodontology (J Periodontol) Vol. 84 Issue 7 Pg. 924-33 (Jul 2013) ISSN: 1943-3670 [Electronic] United States
• PMID: 23003915 (Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Biocompatible Materials
• Calcium Phosphates
• Drug Carriers
• Matrix Metalloproteinase Inhibitors
• beta-tricalcium phosphate
• Edetic Acid
• Doxycycline

Topics

• Acid Etching, Dental (methods)
• Adult
• Alveolar Bone Loss (surgery)
• Biocompatible Materials (chemistry)
• Calcium Phosphates (chemistry)
• Chromatography, High Pressure Liquid
• Chronic Periodontitis (surgery)
• Debridement
• Doxycycline (administration & dosage, analysis, pharmacokinetics)
• Drug Carriers
• Edetic Acid (therapeutic use)
• Female
• Follow-Up Studies
• Gingival Crevicular Fluid (chemistry)
• Humans
• Male
• Matrix Metalloproteinase Inhibitors (administration & dosage, analysis, pharmacokinetics)
• Middle Aged
• Periodontal Attachment Loss (surgery)
• Periodontal Index
• Periodontal Pocket (surgery)
• Single-Blind Method
• Surgical Flaps (surgery)
• Tooth Root (drug effects)
• Treatment Outcome