Abstract |
The pathophysiology of cerebral cortical lesions in multiple sclerosis (MS) is not understood. We investigated cerebral cortex microvessels during immune-mediated demyelination in the MS model chronic murine experimental autoimmune encephalomyelitis (EAE) by immunolocalization of the endothelial cell tight junction (TJ) integral proteins claudin-5 and occludin, a structural protein of caveolae, caveolin-1, and the blood-brain barrier-specific endothelial transporter, Glut 1. In EAE-affected mice, there were areas of extensive subpial demyelination and well-demarcated lesions that extended to deeper cortical layers. Activation of microglia and absence of perivascular inflammatory infiltrates were common in these areas. Microvascular endothelial cells showed increased expression of caveolin-1 and a coincident loss of both claudin-5 and occludin normal junctional staining patterns. At a very early disease stage, claudin-5 molecules tended to cluster and form vacuoles that were also Glut 1 positive; the initially preserved occludin pattern became diffusely cytoplasmic at more advanced stages. Possible internalization of claudin-5 on TJ dismantling was suggested by its coexpression with the autophagosomal marker MAP1LC3A. Loss of TJ integrity was confirmed by fluorescein isothiocyanate-dextran experiments that showed leakage of the tracer into the perivascular neuropil. These observations indicate that, in the cerebral cortex of EAE-affected mice, there is a microvascular disease that differentially targets claudin-5 and occludin during ongoing demyelination despite only minimal inflammation.
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Authors | Mariella Errede, Francesco Girolamo, Giovanni Ferrara, Maurizio Strippoli, Sara Morando, Valentina Boldrin, Marco Rizzi, Antonio Uccelli, Roberto Perris, Caterina Bendotti, Mario Salmona, Luisa Roncali, Daniela Virgintino |
Journal | Journal of neuropathology and experimental neurology
(J Neuropathol Exp Neurol)
Vol. 71
Issue 10
Pg. 840-54
(Oct 2012)
ISSN: 1554-6578 [Electronic] England |
PMID | 23001217
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cav1 protein, mouse
- Caveolin 1
- Claudin-5
- Cldn5 protein, mouse
- Glucose Transporter Type 1
- Occludin
- Ocln protein, mouse
- Slc2a1 protein, mouse
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Topics |
- Animals
- Blood-Brain Barrier
(metabolism, pathology, physiopathology)
- Caveolin 1
(metabolism)
- Cerebral Cortex
(metabolism, pathology, physiopathology)
- Claudin-5
(metabolism)
- Encephalomyelitis, Autoimmune, Experimental
(metabolism, pathology, physiopathology)
- Female
- Glucose Transporter Type 1
(biosynthesis, metabolism)
- Mice
- Mice, Inbred C57BL
- Occludin
(metabolism)
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