Role of neutrophil elastase in lung injury induced by burn-blast combined injury in rats.

Abstract

OBJECTIVE:

Neutrophil elastase (NE) takes part in the pathogenesis of acute lung injury. However, its role in lung injury of burn-blast combined injury is unclear. Our objective was to assess the role of NE, and effect of sivelestat, a specific NE inhibitor, in lung injury induced by burn-blast combined injury in rats.

METHODS:

One hundred and sixty male Sprague-Dawley rats were randomly subjected to burn-blast combined injury (BB) group, burn-blast combined injury plus sivelestat treatment (S) group or control (C) group. Blood gas, protein concentration and NE activity in bronchoalveolar lavage fluid (BALF), pulmonary myeloperoxidase (MPO) activity, serum concentrations of TNF-α and IL-8, etc. were investigated from 0 h to 7 d post-injury.

RESULTS:

In BB group, PaO2 decreased, while NE activity in BALF, total protein concentration in BALF, pulmonary MPO activity and W/D ratio, serum concentrations of TNF-α and IL-8 increased with neutrophil infiltration, progressive bleeding and pulmonary oedema. Compared with BB group, sivelestat treatment decreased the NE activity and ameliorated the above indexes.

CONCLUSION:

Sivelestat, exerts a protective effect in lung injury after burn-blast combined injury through inhibiting NE activity to decrease pulmonary vascular permeability, neutrophil sequestration, and production of TNF-α and IL-8.

• Authors: Jia-ke Chai, Jian-hua Cai, Hu-ping Deng, Xiao-fang Zou, Wei Liu, Qing-gang Hu, Chuan-an Shen, Hui-nan Yin, Xi-bo Zhang, Yun-fei Chi, Li Ma, Rui Feng
• Journal: Burns : journal of the International Society for Burn Injuries (Burns) Vol. 39 Issue 4 Pg. 745-53 (Jun 2013) ISSN: 1879-1409 [Electronic] Netherlands
• PMID: 22999209 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd and ISBI. All rights reserved.

Chemical References

• Interleukin-8
• Proteinase Inhibitory Proteins, Secretory
• Serine Proteinase Inhibitors
• Sulfonamides
• Tumor Necrosis Factor-alpha
• Carbon Dioxide
• sivelestat
• Leukocyte Elastase
• Oxygen
• Glycine

Topics

• Animals
• Blast Injuries (complications, drug therapy, enzymology)
• Bronchoalveolar Lavage Fluid (chemistry)
• Burns (complications, drug therapy, enzymology)
• Carbon Dioxide (metabolism)
• Disease Models, Animal
• Glycine (analogs & derivatives, therapeutic use)
• Interleukin-8 (metabolism)
• Leukocyte Elastase (physiology)
• Lung Injury (drug therapy, enzymology, etiology)
• Male
• Oxygen (metabolism)
• Partial Pressure
• Proteinase Inhibitory Proteins, Secretory (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Serine Proteinase Inhibitors (therapeutic use)
• Sulfonamides (therapeutic use)
• Tumor Necrosis Factor-alpha (metabolism)