The expression of metabolism-related proteins in phyllodes tumors.

Abstract

The purpose of this study was to investigate the association between the expression of hypoxia-inducible factor (HIF)-1α, insulin-like growth factor (IGF)-1, glucose transporter 1 (Glut-1), carbonic anhydrase IX (CAIX), and monocarboxylate transporter (MCT)4, which are metabolism-related proteins in phyllodes tumors (PTs), and clinicopathologic factors and its implication. We used tissue microarrays to analyze 207 PTs and performed immunohistochemical staining against the glycolysis-related molecules HIF-1α, IGF-1, Glut-1, CAIX, and MCT4. We then compared the immunohistochemical results and clinicopathologic parameters. The expressions of HIF-1α, Glut-1, CAIX, and MCT4 in the stromal component of PTs increased (P = 0.019, P < 0.001, P = 0.045, and P < 0.001, respectively) with increasing tumor grade. According to univariate analysis, factors associated with shorter disease-free survival were Glut-1 expression (P = 0.001) and MCT4 expression (P < 0.001) in the stromal component, and the factors associated with shorter overall survival were IGF-1 expression (P = 0.012), Glut-1 expression (P < 0.001), CAIX expression (P = 0.039), and MCT4 expression (P < 0.001) in the stromal component. Our investigation of stromal expression of the metabolism-related proteins HIF-1α, IGF-1, Glut-1, CAIX, and MCT4 revealed that, as the PT grade increased, the stromal expression of HIF-1α, Glut-1, CAIX, and MCT4 significantly increased. This result suggested that increasing PT grade is associated with increased glycolysis in the stromal component.

• Authors: Ji Eun Kwon, Woo-Hee Jung, Ja Seung Koo
• Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 34 Issue 1 Pg. 115-24 (Feb 2013) ISSN: 1423-0380 [Electronic] Netherlands
• PMID: 22986897 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Glucose Transporter Type 1
• Hypoxia-Inducible Factor 1, alpha Subunit
• Monocarboxylic Acid Transporters
• Muscle Proteins
• SLC16A4 protein, human
• Insulin-Like Growth Factor I
• CA9 protein, human
• Carbonic Anhydrase IX
• Carbonic Anhydrases

Topics

• Adult
• Antigens, Neoplasm (metabolism)
• Biomarkers, Tumor (metabolism)
• Breast Neoplasms (metabolism, mortality)
• Carbonic Anhydrase IX
• Carbonic Anhydrases (metabolism)
• Disease-Free Survival
• Energy Metabolism
• Female
• Glucose Transporter Type 1 (metabolism)
• Glycolysis
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Insulin-Like Growth Factor I (metabolism)
• Monocarboxylic Acid Transporters (metabolism)
• Muscle Proteins (metabolism)
• Neoplasm Grading
• Phyllodes Tumor (metabolism)
• Survival