Differential expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in orange-spotted grouper (Epinephelus coioides).

Abstract

Nucleotide-binding oligomerization domain-containing proteins-1 and -2 (NOD1 and NOD2) are members of the NOD-like receptors (NLRs) family. They are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. In this study, the full-length ORF sequences of NOD1 and NOD2 were cloned, and the putative amino acid sequences were identified in orange-spotted grouper (Epinephelus coioides). The complete open reading frame (ORF) of grouper NOD1 contained 2823 bp encoding a 940 amino acid protein. Grouper NOD2 cDNA contained a 2967 bp ORF, encoding a protein of 988 amino acid residues. Both grouper NOD1 and NOD2 had similar domains to human and fish counterparts. Phylogenetic tree analysis showed that grouper NOD1 clustered with grass carp, zebrafish and channel catfish, while NOD2 was most closely related to fugu. Expression patterns of grouper NOD1 and NOD2 were next studied. NOD1 had the highest level of expression in skin while NOD2 in trunk kidney. Post Vibrio alginolyticus (strain EcGS020401), lipopolysaccharide (LPS) or PolyI:C challenges, gene expression of grouper NOD1 and NOD2 was stimulated to different extents. NOD1 showed a significant enhancement after LPS stimulation, but NOD2 increased more significantly after PolyI:C invasion, indicating that NOD1 and NOD2 may exert different effects on the eradication of bacteria and virus. The adaptor protein RIP-like-interacting CLARP kinase (RICK) and downstream molecule interleukin-8 (IL-8) were also induced at different levels after stimulation, which indicated that NOD1 and NOD2 signal transduction was involved in grouper innate immune protection against bacterial and viral infections.

• Authors: Qing-Hua Hou, Shi-Bai Yi, Xu Ding, Hui-Xian Zhang, Yan Sun, Yong Zhang, Xiao-Chun Liu, Dan-Qi Lu, Hao-Ran Lin
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 33 Issue 5 Pg. 1102-11 (Nov 2012) ISSN: 1095-9947 [Electronic] England
• PMID: 22982325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• DNA Primers
• Lipopolysaccharides
• Nod1 Signaling Adaptor Protein
• Nod2 Signaling Adaptor Protein
• Poly I-C

Topics

• Amino Acid Sequence
• Analysis of Variance
• Animals
• Base Sequence
• Cloning, Molecular
• DNA Primers (genetics)
• Gene Expression Profiling
• Gene Expression Regulation (drug effects, immunology)
• Lipopolysaccharides (toxicity)
• Molecular Sequence Data
• Nod1 Signaling Adaptor Protein (genetics, metabolism)
• Nod2 Signaling Adaptor Protein (genetics, metabolism)
• Open Reading Frames (genetics)
• Perciformes (genetics, metabolism)
• Phylogeny
• Poly I-C (toxicity)
• Sequence Analysis, DNA
• Signal Transduction (drug effects, immunology)
• Vibrio alginolyticus (chemistry)