Abstract | BACKGROUND: Omega-3 poly- unsaturated fatty acids (ω-3 PUFAs) have demonstrated to be beneficial in the prevention of cardiovascular disease, however, the mechanisms by which they perform their cardiovascular protection have not been clarified. Intriguingly, some of these protective effects have also been linked to HDL. The hypothesis of this study was that ω-3 PUFAs could modify the protein cargo of HDL particle in a triglyceride non-dependent mode. The objective of the study was to compare the proteome of HDL before and after ω-3 PUFAs supplemented diet. METHODS: A comparative proteomic analysis in 6 smoker subjects HDL before and after a 5 weeks ω-3 PUFAs enriched diet has been performed. RESULTS: CONCLUSIONS: Despite the low number of subjects included in the study, our findings demonstrate that ω-3 PUFAs supplementation modifies lipoprotein containing apoAI (LpAI) proteome and suggest that these protein changes improve the functionality of the particle.
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Authors | Elena Burillo, Rocío Mateo-Gallego, Ana Cenarro, Sarah Fiddyment, Ana M Bea, Inmaculada Jorge, Jesús Vázquez, Fernando Civeira |
Journal | Lipids in health and disease
(Lipids Health Dis)
Vol. 11
Pg. 116
(Sep 16 2012)
ISSN: 1476-511X [Electronic] England |
PMID | 22978374
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- APOA1 protein, human
- Apolipoprotein A-I
- CLU protein, human
- Cardiotonic Agents
- Clusterin
- Fatty Acids, Omega-3
- Lipoproteins, HDL
- Proteome
- Aryldialkylphosphatase
- PON1 protein, human
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Topics |
- Adult
- Apolipoprotein A-I
(blood, isolation & purification)
- Aryldialkylphosphatase
(blood, isolation & purification)
- Cardiotonic Agents
(administration & dosage)
- Cardiovascular Diseases
(blood, etiology, prevention & control)
- Chromatography, Affinity
- Clusterin
(blood, isolation & purification)
- Dietary Supplements
- Fatty Acids, Omega-3
(administration & dosage)
- Humans
- Lipoproteins, HDL
(blood, isolation & purification)
- Male
- Middle Aged
- Proteome
(isolation & purification, metabolism)
- Smoking
(adverse effects, blood)
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