Abstract | PURPOSE: METHODS: Female Sprague-Dawley rats underwent concomitant right nephrectomy and left renal hilar occlusion for 30 minutes. Twelve animals were equally divided into three groups: Group 1 received no pharmacologic pretreatment, group 2 was pretreated with zaprinast 10 mg/kg, and group 3 was pretreated with zaprinast 20 mg/kg. Zaprinast was dissolved in 25% dimethyl sulfoxide and given as a single intraperitoneal injection 30 minutes before surgery. Serum blood urea nitrogen (BUN) and creatinine levels, histopathology, and TUNEL staining for apoptosis were assessed 24 hours postoperatively. RESULTS: The mean creatinine level for groups 1, 2, and 3 was 0.73 mg/dL, 0.55 mg/dL, and 0.38 mg/dL, respectively. These values were not statistically different (P=0.099). The mean BUN levels of 35.8 mg/dL for group 1, 27.3 mg/dL for group 2, and 23.3 mg/dL for group 3 were also statistically similar (P=0.278). There were no objective differences in histopathologic evaluation or TUNEL staining between the groups. CONCLUSION: This study did not demonstrate a beneficial effect of zaprinast pretreatment on renal parameters after warm ischemic injury.
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Authors | Anthony J Polcari, Ahmer V Farooq, Michael E Woods, Matthew S Ripsch, Maria Picken, Thomas M T Turk, Fletcher A White |
Journal | Journal of endourology
(J Endourol)
Vol. 27
Issue 3
Pg. 338-42
(Mar 2013)
ISSN: 1557-900X [Electronic] United States |
PMID | 22974473
(Publication Type: Journal Article)
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Chemical References |
- Phosphodiesterase 5 Inhibitors
- Purinones
- Creatinine
- zaprinast
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Topics |
- Animals
- Blood Urea Nitrogen
- Creatinine
(blood)
- Female
- In Situ Nick-End Labeling
- Phosphodiesterase 5 Inhibitors
(pharmacology, therapeutic use)
- Purinones
(pharmacology, therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(blood, drug therapy, enzymology, pathology)
- Staining and Labeling
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