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Effect of the phosphodiesterase-5 inhibitor zaprinast on ischemia-reperfusion injury in rats.

AbstractPURPOSE:
The cardiac and renal protective effects of phosphodiesterase-5 (PDE-5) inhibitors against ischemia-reperfusion injury have recently been demonstrated in animal studies. We evaluated the effect of pretreatment with the PDE-5 inhibitor zaprinast on warm renal ischemia in a rat model.
METHODS:
Female Sprague-Dawley rats underwent concomitant right nephrectomy and left renal hilar occlusion for 30 minutes. Twelve animals were equally divided into three groups: Group 1 received no pharmacologic pretreatment, group 2 was pretreated with zaprinast 10 mg/kg, and group 3 was pretreated with zaprinast 20 mg/kg. Zaprinast was dissolved in 25% dimethyl sulfoxide and given as a single intraperitoneal injection 30 minutes before surgery. Serum blood urea nitrogen (BUN) and creatinine levels, histopathology, and TUNEL staining for apoptosis were assessed 24 hours postoperatively.
RESULTS:
The mean creatinine level for groups 1, 2, and 3 was 0.73 mg/dL, 0.55 mg/dL, and 0.38 mg/dL, respectively. These values were not statistically different (P=0.099). The mean BUN levels of 35.8 mg/dL for group 1, 27.3 mg/dL for group 2, and 23.3 mg/dL for group 3 were also statistically similar (P=0.278). There were no objective differences in histopathologic evaluation or TUNEL staining between the groups.
CONCLUSION:
This study did not demonstrate a beneficial effect of zaprinast pretreatment on renal parameters after warm ischemic injury.
AuthorsAnthony J Polcari, Ahmer V Farooq, Michael E Woods, Matthew S Ripsch, Maria Picken, Thomas M T Turk, Fletcher A White
JournalJournal of endourology (J Endourol) Vol. 27 Issue 3 Pg. 338-42 (Mar 2013) ISSN: 1557-900X [Electronic] United States
PMID22974473 (Publication Type: Journal Article)
Chemical References
  • Phosphodiesterase 5 Inhibitors
  • Purinones
  • Creatinine
  • zaprinast
Topics
  • Animals
  • Blood Urea Nitrogen
  • Creatinine (blood)
  • Female
  • In Situ Nick-End Labeling
  • Phosphodiesterase 5 Inhibitors (pharmacology, therapeutic use)
  • Purinones (pharmacology, therapeutic use)
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury (blood, drug therapy, enzymology, pathology)
  • Staining and Labeling

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