Abstract |
The objective of this study is to evaluate the promoter methylation status of the p14ARF in esophageal squamous cell carcinoma (ESCC). Cell lines were treated with the demethylation agent 5-aza-2'-deoxycytidine, and p14ARF messenger RNA ( mRNA) expression was detected by reverse transcription-polymerase chain reaction. We analyzed the methylation status of p14ARF promoter by methylation-specific polymerase chain reaction in 50 ESCC and their noncarcinoma tissues. Then demethylation caused by 5-aza-2'-deoxycytidine increased the p14ARF mRNA expression level in esophagus cancer cell lines. p14ARF methylation was found in 48% (24 of 50) of ESCC patients but only in 18% (9 of 50) corresponding noncarcinoma tissues (P = 0.001). There was a statistically significant correlation between the presence of methylation and tumor metastasis (P < 0.001). The p14ARF mRNA was lower in ESCC tissues than nontumor tissues (mean ± standard deviation, 0.47 ± 0.32 vs. 1.40 ± 0.58; P = 0.002). Meanwhile, a signification association was found between the methylation status of p14ARF promoter and p14ARF mRNA expression in tissues (P < 0.05). The aberrant promoter methylation of p14ARF is a common phenomenon in ESCC, which may be an important mechanism of downregulating p14ARF mRNA expression.
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Authors | Y Ling, C Zhang, R Shen, Y Xu, C Zhu, M Lu, Y Liu, C Zhang |
Journal | Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus
(Dis Esophagus)
2014 Feb-Mar
Vol. 27
Issue 2
Pg. 182-7
ISSN: 1442-2050 [Electronic] United States |
PMID | 22973996
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 Copyright the Authors. Journal compilation © 2012, Wiley Periodicals, Inc. and the International Society for Diseases of the Esophagus. |
Chemical References |
- RNA, Messenger
- Tumor Suppressor Protein p14ARF
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Topics |
- Base Sequence
- Carcinoma, Squamous Cell
(genetics)
- Cell Line, Tumor
- DNA Methylation
(genetics)
- Esophageal Neoplasms
(genetics)
- Esophageal Squamous Cell Carcinoma
- Female
- Gene Expression Regulation, Neoplastic
- Genes, p16
- Humans
- Male
- Molecular Sequence Data
- Promoter Regions, Genetic
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Suppressor Protein p14ARF
(genetics, metabolism)
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