High-throughput screening of stem cell therapy for globoid cell leukodystrophy using automated neurophenotyping of twitcher mice.

Abstract

Globoid cell leukodystrophy (Krabbe's disease) is an autosomal recessive neurodegenerative disorder that results from the deficiency of galactosylceramidase, a lysosomal enzyme involved in active myelination. Due to the progressive, lethal nature of this disease and the limited treatment options available, multiple laboratories are currently exploring novel therapies using the mouse model of globoid cell leukodystrophy. In order to establish a protocol for motor function assessment of the twitcher mouse, this study tested the capability of an automated system to detect phenotypic differences across mouse genotypes and/or treatment groups. The sensitivity of this system as a screening tool for the assessment of therapeutic interventions was determined by the administration of murine bone marrow-derived stem cells into twitcher mice via intraperitoneal injection. Animal behavior was analyzed using the Noldus EthoVision XT7 software. Novel biomarkers, including abnormal locomotion (e.g., velocity, moving duration, distance traveled, turn angle) and observed behaviors (e.g., rearing activity, number of defecation boli), were established for the twitcher mouse. These parameters were monitored across all mouse groups, and the automated system detected improved locomotion in the treated twitcher mice based on the correction of angular velocity, turn angle, moving duration, and exploratory behavior, such as thigmotaxis. Further supporting these findings, the treated mice showed improved lifespan, gait, wire hang ability, twitching severity and frequency, and sciatic nerve histopathology. Taken together, these data demonstrate the utility of computer-based neurophenotyping for motor function assessment of twitcher mice and support its utility for detecting the efficacy of stem cell-based therapy for neurodegenerative disorders.

• Authors: Brittni A Scruggs, Annie C Bowles, Xiujuan Zhang, Julie A Semon, Evan J Kyzar, Leann Myers, Allan V Kalueff, Bruce A Bunnell
• Journal: Behavioural brain research (Behav Brain Res) Vol. 236 Issue 1 Pg. 35-47 (Jan 01 2013) ISSN: 1872-7549 [Electronic] Netherlands
• PMID: 22951180 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Green Fluorescent Proteins

Topics

• Animals
• Behavior, Animal (physiology)
• Body Weight (physiology)
• Green Fluorescent Proteins (genetics)
• Grooming
• High-Throughput Screening Assays
• Injections, Intraperitoneal
• Leukodystrophy, Globoid Cell (pathology, psychology, therapy)
• Life Expectancy
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells
• Mice
• Mice, Inbred C57BL
• Mice, Neurologic Mutants
• Mice, Transgenic
• Motor Activity (physiology)
• Phenotype
• Psychomotor Performance (physiology)
• Stem Cell Transplantation (methods)