HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Escherichia coli Nissle 1917 targets and restrains mouse B16 melanoma and 4T1 breast tumors through expression of azurin protein.

Abstract
Many studies have demonstrated that intravenously administered bacteria can target and proliferate in solid tumors and then quickly be released from other organs. Here, we employed the tumor-targeting property of Escherichia coli Nissle 1917 to inhibit mouse B16 melanoma and 4T1 breast tumors through the expression of azurin protein. For this purpose, recombinant azurin-expressing E. coli Nissle 1917 was developed. The levels of in vitro and in vivo azurin secretion in the engineered bacterium were determined by immunochemistry. Our results demonstrated that B16 melanoma and orthotopic 4T1 breast tumor growth were remarkably restrained and pulmonary metastasis was prevented in immunocompetent mice. It is worth noting that this therapeutic effect partially resulted from the antitumor activity of neutrophils and lymphocytes due to inflammatory responses caused by bacterial infections. No toxicity was observed in the animal during the experiments. This study indicates that E. coli Nissle 1917 could be a potential carrier to deliver antitumor drugs effectively for cancer therapy.
AuthorsYunlei Zhang, Youming Zhang, Liqiu Xia, Xiangli Zhang, Xuezhi Ding, Fu Yan, Feng Wu
JournalApplied and environmental microbiology (Appl Environ Microbiol) Vol. 78 Issue 21 Pg. 7603-10 (Nov 2012) ISSN: 1098-5336 [Electronic] United States
PMID22923405 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents
  • Azurin
Topics
  • Animals
  • Antineoplastic Agents (therapeutic use)
  • Azurin (biosynthesis, genetics, therapeutic use)
  • Cell Line, Tumor
  • Disease Models, Animal
  • Drug Delivery Systems
  • Escherichia coli (classification, genetics, metabolism)
  • Female
  • Genetic Engineering
  • Lung Neoplasms (prevention & control, secondary)
  • Mammary Neoplasms, Experimental (metabolism, microbiology, therapy)
  • Melanoma, Experimental (metabolism, microbiology, therapy)
  • Mice
  • Mice, Inbred BALB C
  • Plasmids

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: