Knockdown of zinc finger protein X-linked inhibits prostate cancer cell proliferation and induces apoptosis by activating caspase-3 and caspase-9.

Zinc finger protein X-linked (ZFX) is a highly conservative mammalian gene with related functions in cell survival and proliferation. However, there are limited reports on regulation of ZFX as a therapeutic target in cancer treatment. In this study, the expression of ZFX in prostate cancer with matched normal adjacent tissues (n=45) and benign prostatic hyperplasia (BPH) tissues (n=16) were observed by immunohistochemical analysis. The effect of lentiviral siRNA (small interference RNA)-mediated dysfunction of ZFX on the proliferation of prostate cancer cells was studied. ZFX mRNA and protein expression levels in prostate cancer cells (PC-3 and DU145) were analyzed by western blotting and real-time polymerase chain reaction (RT-PCR). The effects of siRNA targeting ZFX on growth, cell cycle and apoptosis of PC-3 cells were evaluated by MTT assay and flow cytometry. We also investigated the effect of ZFX deletion on the activation of caspase-1, -3 and -9 by western blotting and colorimetric assay. Prostate cancer specimens appeared significantly higher (42.2% of cases being positive) than that observed in normal adjacent tissues (11.8% of cases being positive) and BPH tissues (12.5% of cases being positive). Repression of ZFX in the prostate cancer cells effectively suppressed the cellular proliferation and colony-formation ability, and led to G1 phase cell cycle arrest. Moreover, inhibition of ZFX-induced cell apoptosis by activating caspase-1, -3 and -9. In conclusion, ZFX represents the prominent role in the progression of prostate cancer and may be a promising therapy target for prostate cancer.
AuthorsH Jiang, L Zhang, J Liu, Z Chen, R Na, G Ding, H Zhang, Q Ding
JournalCancer gene therapy (Cancer Gene Ther) Vol. 19 Issue 10 Pg. 684-9 (Oct 2012) ISSN: 1476-5500 [Electronic] England
PMID22898899 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Kruppel-Like Transcription Factors
  • zinc finger protein, X-linked
  • Caspase 3
  • Caspase 9
  • Apoptosis (physiology)
  • Caspase 3 (genetics, metabolism)
  • Caspase 9 (genetics, metabolism)
  • Cell Growth Processes (physiology)
  • Enzyme Activation
  • Gene Knockdown Techniques
  • Humans
  • Immunohistochemistry
  • Kruppel-Like Transcription Factors (biosynthesis, deficiency, genetics, metabolism)
  • Male
  • Prostatic Neoplasms (enzymology, genetics, pathology, therapy)
  • Zinc Fingers

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password:
Type Validation Code Shown: