Abstract | STUDY QUESTION: SUMMARY ANSWER:
Pravastatin does not prevent the effects of aPL on human first trimester trophoblast cell function. WHAT IS KNOWN ALREADY:
Antiphospholipid syndrome (APS) is associated with recurrent pregnancy loss and late pregnancy complications, such as pre-eclampsia, owing to direct targeting of the placenta by aPL. While treatment with heparin reduces the rate of pregnancy loss, the risk for severe pre-eclampsia remains high. Thus, there is a need to find alternative treatments for the prenatal management of patients with APS. Statins have recently been shown to prevent aPL-mediated fetal loss in mice but their effects on a human pregnancy model of APS have not yet been studied. DESIGN, DATA COLLECTION, METHODS: MAIN FINDINGS: Using the human first trimester trophoblast cell line, HTR8, pravastatin significantly augmented, compared with no treatment, aPL-dependent secretion of interleukin (IL)-8 (P< 0.05), IL-1β (P< 0.05) and soluble endoglin (P< 0.01) but had no effect on aPL-induced up-regulation of vascular endothelial growth factor, placenta growth factor or growth-related oncogene alpha secretion. Furthermore, pravastatin alone limited basal HTR8 cell migration (P< 0.01), and did not mitigate the adverse effect of aPL on trophoblast migration. Pravastatin also had no impact on the secretion of pro-inflammatory cytokines and angiogenic factors by primary human first trimester trophoblast cells exposed to aPL. LIMITATIONS AND WIDER IMPLICATIONS OF THE FINDINGS: STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the American Heart Association.
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Authors | Ebelechukwu A Odiari, Melissa J Mulla, Anna K Sfakianaki, Michael J Paidas, Nancy L Stanwood, Aileen Gariepy, Jan J Brosens, Larry W Chamley, Vikki M Abrahams |
Journal | Human reproduction (Oxford, England)
(Hum Reprod)
Vol. 27
Issue 10
Pg. 2933-40
(Oct 2012)
ISSN: 1460-2350 [Electronic] England |
PMID | 22888169
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenesis Inducing Agents
- Antibodies, Antiphospholipid
- Antibodies, Monoclonal
- Cytokines
- beta 2-Glycoprotein I
- Pravastatin
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Topics |
- Angiogenesis Inducing Agents
(metabolism)
- Antibodies, Antiphospholipid
(immunology)
- Antibodies, Monoclonal
- Antiphospholipid Syndrome
(drug therapy, immunology)
- Cell Line
- Cell Movement
(drug effects)
- Cytokines
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Humans
- Multiplex Polymerase Chain Reaction
- Pravastatin
(pharmacology)
- Trophoblasts
(drug effects, immunology, pathology)
- beta 2-Glycoprotein I
(immunology)
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